肝脏射频消融对大鼠外周血单个核细胞OX62,OX6及CD86表达的影响(英文)

Effects of radiofrequency ablation on OX62, OX6 and CD86expression in peripheral blood mononuclear cells of the rat liver

背景:既往已有研究证实经皮射频消融在有效灭活肿瘤的同时,能够在一定程度上改善机体免疫抑制状态。抗原呈递细胞在机体的抗肿瘤免疫中发挥着重要的作用,树突状细胞是目前所知机体内功能最强的专职性抗原提呈细胞。目的:观察肝脏射频消融后大鼠外周血单个核细胞OX-62,OX-6和CD86的表达变化。设计、时间及地点:随机对照动物实验,于2005-06/2006-03在北京大学临床肿瘤学院完成。材料:选用SD大鼠12只,按随机数字表法分为2组,对照组和射频后1周组各6只。方法:对照组大鼠处死后抽取外周血2mL。将射频后1周组大鼠肝脏左叶牵出,斜置电极针入肝,展开电极针,射频作用时间4min。射频完毕给予抗感染治疗,射频治疗后1周处死后抽取外周血。主要观察指标:①正常鼠肝射频后大体和病理学改变。②射频前后外周血内单个核细胞OX-62,OX-6以及CD86表达水平。结果:鼠肝组织射频灶病理组织学检查显示从中心到外围呈凝固性坏死-细胞变性-肉芽组织形成的演变特点。射频后1周组大鼠外周血单个核细胞中树突状细胞特异性标志OX-62的阳性表达率为(0.70±0.16)%,显著高于对照组[(0.34±0.08)%,P<0.05]。2组大鼠外周血树突状细胞中OX-6,CD86的阳性表达率差异无显著性意义(P>0.05)。结论:射频消融可促进大鼠外周血中树突状细胞前体细胞数量增多,可能会对提高机体在免疫应答中的抗原提呈能力起到促进作用。

BACKROUND： Previous studies have confirmed that radiofrequency ablation （RFA） has not only efficiently killed tumor cells, but also improved immune suppression of organism. Antigen presenting cells play an important role during anti-tumor immune reaction. Dendritic cells are most powerful antigen presenting cells. OBJECTIVE： To study the influence of RFA on OX-62, OX-6 and CD86 expression of rat peripheral blood mononuclear cells. DESIGN, TIME AND SETTING： The randomized controlled animal observation was performed at the School of Oncology of Peking University from June 2005 to March 2006. MATERIALS： A total of 12 Sprague Dawley rats were equally and randomly assigned into control and 1-week radiofrequency groups. METHODS： 2 mL peripheral blood was extracted from rats of the control group following rats weresacrificed. The left lobe of rat liver was exposed, inserted with acicular electrodes at tilted position. The acicular electrodes were spread out. The action time was 4 minutes. After RFA, the rats were given anti-infective therapy. 2 mL peripheral blood was taken out after they were put to death 1 week after RFA. MAIN OUTCOME MEASURES： The following parameters were measured： pathomorphological observation on the liver of normal rats after RFA; OX-62, OX-6 and CD86 expression of peripheral blood mononuclear cells before and after RFA. RESULTS： Pathologyical examination after RFA showed the characteristic that coagulation necrosis and cellular degeneration and granulation tissue forming appeared from target center to peripheral of the target. Positive expression rate of OX-62 in rat peripheral blood in the 1-week radiofrequency group [（0.70±0.16）%] was significantly higher than in the control group [（0.34±0.08）%, P 〈 0.05]. No significant difference in positive expression rate of OX-6 and CD86 in the peripheral blood mononuclear cells between both groups （P 〉 0,05）. CONCLUSION： RFA can promote the increased number of precursor Dendritic cells in rat peripheral blood, which may be contributed to improve the ability to angtigen presentation during immune response.