摘要
研究的目的是在胃的癌房间学习人的 yrdC 基因的功能的目的。方法人的 yrdC 基因被 RT-PCR 从人的脾组织孤立。反人的 yrdC 单音的同种细胞的抗体被 hybridoma 准备房间技术。带 yrdC 基因的 Recombinant 侵入人体气管粘膜的病菌 Ad.yrdC 被使用 AdEasy adenoviral 向量系统构造。Recombinant 侵入人体气管粘膜的病菌 Ad.yrdCshRNA 调停了 yrdCshRNA 被 RNA 干扰技术准备。中等区别的胃的腺癌 BGC-823 房间是 transfected， transfected 房间的吸收度在由甲基 thiazolyl tetrazolium (MTT ) 的 490 nm 是计算的方法。transfected Ad.yrdC 组的结果 A 价值比 non-transfected 和 transfected 广告的显著地大。Ad.yrdCshRNA 的空组，和价值组织比 non-transfected 和 transfected 广告的显著地低。空组。yrdC 基因的结论表示有支持胃的癌房间的增长的功能。
Objective： The aim of the research was to study the function of human yrdC gene in the gastric carcinoma cells. Methods： Human yrdC gene was isolated from human spleen tissue by RT-PCR. Anti-human yrdC monoclonal antibody was prepared by hybridoma cell technique. Recombinant adenovirus Ad.yrdC carrying yrdC gene was constructed by using the AdEasy adenoviral vector system. Recombinant adenovirus Ad.yrdCshRNA mediated yrdCshRNA was prepared by RNA interference technology. Gastric adenocarcinoma BGC-823 cells of moderate differentiation were transfected and absorbance of the transfected cells was calculated at 490 nm by methyl thiazolyl tetrazolium （MTT） method. Results： A value of the transfected Ad.yrdC group was significantly greater than that of the non-transfected and transfected Ad.Null groups, and A value of Ad.yrdCshRNA group was significantly lower than that of the non-transfected and transfected Ad.Null groups. Conclusion： Expression of yrdC gene has a function of promoting the proliferation of gastric carcinoma cells.
基金
Supported by a grant from the General Program of National Natural Science Foundation of China （No, 30371403）.
