摘要
Objective： The aim of this study was to study the inhibition effect of small interfering RNAs （siRNA） on gene expression in MG-63 cells,and to study the inhibitory effect on metastasis of MG-63. Methods： A plasmid of a short hairpin RNA targeting acid phosphatase 1 （ACP1） was constructed and transfected into MG-63 cell line. ACP1 expression of MG-63 cells before and after transfection was detected by RT-PCR and Western blot. The capacity of adhesion, migration and inva- sion was examined by adhesion assay, migration assay and transwell assay. Results： The recombinant plasmid pGenesil-l/ ACPI-shRNA was successfully constructed, shRNA efficiently inhibited the expression of ACPI by gene and protein level and suppressed cell migration. The adhesion decreased from 96.41±8.83 to 43.38 ± 6.03 （P 〈 0.01）, invasion ability from 56.5 ± 4.8 to 36.3 ± 6.1 （P 〈 0.05）. Conclusion： Down-regulating ACP1 by shRNA reduced the capacity of metastasis of MG-63 cell,which providing a novo-approach to biotherapy of cancer.
