CD4^＋CD25^＋/highCD127^low在传染性单核细胞增多症患儿中的“永生细胞”抑制作用

Study of the inhibition effects of CD4 ^＋ CD25^ ＋/highCD127^low on the immortalized cells in children with infectious mononucleosis

目的探讨CD4^＋CD25^＋/highCD127^low在传染性单核细胞增多症（IM）EB病毒（EBV）转化的“永生细胞”抑制途径的作用。方法流式细胞仪测定23例急性期IM患儿和其中10例恢复期随访EBV感染IM患儿CD3^＋、CD3^＋CD4^＋、CD3^＋CD8^＋、CD4^＋CD25^＋/highCD127^low和CD19^＋、CD19^＋CD23^＋表达情况，并与20例正常对照组比较。结果IM患儿CD3^＋、CD3^＋CD8^＋与正常对照组比较有明显升高，而CD3^＋CD4^＋、CD4^＋／CD8^＋比值、CD4^＋CD25^＋/highCD127^low、CD19^＋、CD19^＋CD23^＋明显低于正常对照组。CD3^＋、CD3^＋CD8^＋急性期明显高于恢复期，CD3^＋CD4^＋、CD4^＋／CD8^＋比值、CD4^＋CD25^＋/highCD127^low、CD19^＋、CD19^＋CD23^＋恢复期都明显下降。在疾病恢复期，CD3^＋CD8^＋、CD4^＋／CD8^＋比值、CD19^＋、CD19^＋CD23^＋仍有显著性差异，其他指标与正常对照组之间差异无统计学意义。在急性期CD3^＋、CD3^＋CD8^＋与CD19^＋、CD19^＋CD23^＋细胞均呈负相关，而CD4^＋CD25^＋/highCD127^low与CD19^＋、CD19^＋CD23^＋细胞均呈正相关。结论在EBV感染儿童IM发病过程中，CD4^＋CD25^＋/highCD127^low的降低可能对EBV转化的“永生细胞”在体内的有效抑制起着主要的作用。

Objective To explore the inhibition effects of CD4^＋ CD25^ ＋/highCD127^low on the EBV immortalized cells（ CD23 ^＋ ）in children with infectious mononucleosis （IM）. Methods The expression of CD3^ ＋ , CD3^ ＋ CD4^＋ , CD3^ ＋ CD8^＋ , CD4^＋ CD25^ ＋/high CD^127low and CD19^＋ , CD19^＋ CD23 ^＋ were analyzed by flow cytometry in 23 children at the acute stage of IM and 10 children recovering from IM in comparison with the ones of 20 healthy controls. Results Compared with the following results in controls, CD3^ ＋ , CD3^ ＋ CD8^＋ were significantly increased and CD3^ ＋ CD4^＋, CD4^＋/CD8^＋ ratio, CD4^＋ CD25 ＋/highCD^127low, CD19^＋ , CD19^＋ CD23 ^＋ were significantly reduced in the IM patients. Compared with the ones in the IM patients recovering from IM, CD3^ ＋ , CD3^ ＋ CD8^＋ were significantly increased and CD3^ ＋ CD4^ ＋ , CD4^＋/CD8^＋ ratio, CD4^＋ CD25 ^＋/highCD^127^low, CD19^＋, CD19^ ＋ CD23 ^＋ were significantly reduced in the IM patients at the acute stage. There was no obvious difference in all the marks except CD4^＋/CD8^＋ ratio, CD8^＋ CD28 ^＋ , CD19^＋ , CD19^＋ CD23 ^＋ ratio between the children recovering from IM and the normal controls. There is a positive correlation between CD3^ ＋ and CD19^＋ , CD19^＋ CD23 ^＋ during acute phase. There is a negative correlation between CD4^＋ CD25^ ＋/hishCD^127low and CD19^＋ , CD19^＋ CD23 ^＋ during acute phase. Conclusion The results indicate that the decreasing of CD4^＋ CD25 ^＋/highCD127^low may play an important role in eliminating EBV immortalized cells.