活动性肺结核患者基质金属蛋白酶-9和血管内皮细胞生长因子在血清中的表达及其临床意义

Investigation of the serum MMP-9 and VEGF and its role in active pulmonary tuberculosis patients

目的：探讨基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）及血管内皮细胞生长因子（vascular endothelial growth factor，VEGF）在活动性肺结核患者中的表达及其临床意义。方法：采用ELISA方法检测42例活动性肺结核患者及42例健康人血清中MMP-9、VEGF表达水平。结果：活动性肺结核患者血清MMP-9、VEGF水平显著高于健康对照者（P〈0．001）；痰结核杆菌涂片阳性患者血清中MMP-9、VEGF水平显著高于涂片阳性患者（P〈0．001）；肺内广泛病变患者血清中MMP-9、VEGF水平显著高于肺内局限病变患者（P〈0．001）。结论：MMP-9、VEGF的表达水平可能与活动性肿结核的发生发展有关，并且与肺结核临床特点密切相关。

Objective To study the relationship between the serum expression of MMP-9 and VEGF with the clinical characteristics in active pulmonary tuberculosis patients. Methods Expression of MMP-9 and VEGF were detected by ELISA method in 42 patients with active pulmonary tuberculosis and with a control of 42 health cases. Results The serum levels of MMP-9 and VEGF in active pulmonary tuberculosis patients were significantly higher than that of healthy group（P〈0. 001）. The serum levels of MMP--9 and VEGF in patients with advanced disease, sputum positive patients and pulmonary cavity patients were significantly higher than that in those with limited disease, sputum negative and without cavity respectively in active pulmonary tuberculosis patients （P〈0. 001）. Conclusion： Our data suggest the serum levels of MMP-9 and VEGF play an important role in active pulmonary tuberculosis patients and have close relation with clinical characteristics in active pulmonary tuberculosis patients.