芪棱汤对局灶性脑缺血再灌注损伤大鼠5-BrdU蛋白表达的影响

Effects of Qileng Decoction on the Expression of 5-BrdU protein in Rats with Focal Cerebral Ischemia-Reperfusion Injury

目的：探讨芪棱汤对局灶性脑缺血再灌注大鼠5-Brdu蛋白表达的影响。方法：将大鼠随机分为假手术组、模型组、芪棱汤去养阴药组及芪棱汤组。采用颈内动脉线栓加环扎法复制大鼠局灶性脑缺血再灌注模型（MCAO＼Reperfusion）。在再灌注后不同时间点采用免疫组化法检测5-BrdU蛋白表达。结果：芪棱汤去养阴药汤组与芪棱汤组5-BrdU蛋白表达明显多于模型组（P〈0．05），且芪棱汤5-BrdU蛋白表达明显多于芪棱汤去养阴药汤组（P〈0．05）。结论：芪棱汤能够促进5-Brdu蛋白表达，其可能通过刺激内源性神经干细胞增殖达到神经保护作用。

Objective：To study the effect of the expression of 5--BrdU protein in Qileng decoction on brain after focal cerebral ischemia reperfusion rat. Method：The rats were randomly divided into 4 groups including the sham--operation group, the MCAO （Middle Cerebral Artery Occlusion） group, the group of Qileng Decoction without nourishing--Yin herbs and the Qileng Decoction group, the MCAO model was established with method of internal carotid artery occlusion by suture. 5--BrdU were observed with immunohistochemistry method at different reperfusion time. Result： The expression of 5 -- BrdU protein in model group was significantly lower than that in both Qileng Decoction without nourishing-- Yin herbs group and QiLeng decoction group （P〈0. 05）, and the expression of 5--BrdU in QiLeng decoction group were increased significantly compared with the group of Qileng Decoction without nourishing--Yin herbs（P〈0. 05）. Concolusion： QiLeng decoction play the neuron protective effects by increasing the expression of the 5--BrdU protein.