吸烟者与非吸烟者肺泡灌洗液中肺表面活性蛋白A和D含量的比较

The contents of Surfactant Protein A and D in BAL Fluids of Healthy Smokers and Healthy Nonsmokers

目的探讨吸烟对肺表面活性蛋白A（SP-A）和肺表面活性蛋白D（SP-D）含量的影响。方法通过双抗体夹心ELISA法测定11名吸烟者和13非吸烟者肺泡灌洗液中的SP-A和SP-D的含量。结果非吸烟者肺泡灌洗液中SP-A和SP-D含量均明显高于吸烟者[（3．1±0．40）μg/ml与（1．8±0．4）μg／ml，（1．5±0．2）μg／ml与（0．6±0．1）μg／ml，均P〈0．05]。结论吸烟者SP—A和SP-D水平的降低可能损害宿主的肺泡免疫功能，而且可能对慢性阻塞性肺疾病的产生起重要作用。

Objective To observe whether cigarette smoke alter the component of pulmonary surfactant protein A and D. Methods In this study, we determined the contents of SP-A and SP-D in BAL fluids of healthy smokers and nonsmokers by enzyme linked immunosorbent assay（ELISA）. Resluts The contents of SP-A and SP-D in BAL fluids were significantly decreased in smokers compared to those in nonsmokers [ （3.1 ± 0. 40） μg/ml vs （ 1.8 ± 0. 4） μg/ml, （ 1.5 ±0. 2 ） μg/ml vs （0. 6 ± 0. 1 ） μg/m, all P 〈 0. 05 ]. Conclusion These results suggested that the decreased levels of SP-A and SP-D in smokers may impair the host defense functions of surfactant in the peripheral airways and might have a crucial role in the development of chronic obstructive lung disease.