阴阳平衡散灌肠对溃疡性结肠炎患者CD4^＋CD25^＋的影响

Effects and mechanism of CD4^＋ CD25^＋ regulatory T cells in colitis treated by Yin Yang equilibrated preparation

目的探讨蚂蚁制剂阴阳平衡散灌肠治疗UC的作用机制。方法溃疡性结肠炎患者82例，其中阴阳平衡散灌肠组（A组）40例和柳氮磺氨吡啶（SASP）栓剂塞肛治疗组（B组）42例，健康对照组35例，用流式细胞仪检测各组外周血及肠黏膜组织中CD4^＋CD25^＋Tregs细胞的变化。结果治疗前UC患者外周血及肠黏膜组织中的CD4^＋CD25^＋Tregs细胞数量均显著低于健康对照组（P〈0.01），而经SASP和阴阳平衡散灌肠治疗后该细胞亚群明显高于治疗前及对照组（P〈0．01或P〈0．05）。结论CD4^＋、CD25^＋Tregs细胞亚群在UC的发病及病情转归中可能具有重要免疫调节作用，阴阳平衡散通过调节Tregs细胞的数量对UC发挥治疗作用。

Objective To explore the effects and mechanism in patients with ulcerative colitis （UC） treated by Yin Yang equilibrated preparation. Method 82 patients with UC as treatment group and 35 health persons as control group underwent the testing. The serum and mueosal of all testing persons were taken pre and post treatment to detect CD4^＋ CD25^＋ regulatory T cells （Tregs） by flow cytometry at the end of one month, treating with SASP and Yin Yang equilibrated preparation. Resu/t Compared with normal control group,the proportion of CD4^＋ CD25^＋ Tregs was markedly decreased in PB and mucosal of group A and B （ P 〈 0. 01 ）. But it was significantly increased in therapeutic groups of SASP and Yin Yang equilibrated preparation, and their CD; CD25^＋ Tregs in PB and mucosal were much more than the control group at the end of one month after treating with SASP and Yin Yang equilibrated preparation（ P 〈 0. 01 or P 〈 0. 05）. Conclusion CD4^＋ CD25^＋ Tregs with strong immune suppression play a central role in the initiation and development of UC,and the Yin Yang equilibrated preparation might exert its therapeutic effects on UC by the regulation of number and function of CD4^＋ CD25^＋ Tregs.