摘要
目的探讨人参总皂苷(TSPG)对人白血病细胞株K562生长和凋亡的影响及其可能机制。方法采用噻唑兰比色法(Mar)观察TSPG对K562细胞生长的影响;流式细胞仪观察TSPG对细胞凋亡的影响;用RT-PCR检测TSPG诱导K562细胞凋亡中生存素(Survivin)基因的表达情况。结果TSPG对K562细胞生长有抑制作用,呈剂量依赖关系(P〈0.05);10、100、200μg/ml组细胞凋亡率分别为16.67%、23.78%、33.98%,药物组与对照组差异有统计学意义(P〈0.01或P〈0.05);随着TSPG浓度升高,Survivin基因的表达水平逐渐下调(P〈0.05)。结论TSPG可以抑制人白血病细胞的生长并诱导其凋亡,其机制可能与下调Survivin基因的表达有关。
Objective To study the effects of TSPG on apoptosis of K562 cells and the probable mechanism involved. Methods MTT was used to investigate the proliferation of K562 cells; Flow cytometry (FCM) was used to investigate the effects of TSPG on apoptosis of K562 cells ; The expression of Survivin in K562 cells treated with different concentraction of TSPG were examined by RT-PCR. Results The growth of K562 cells was inhibited by TSPG in the concentration dependent manner (P 〈 0. 05 ). FCM showed that the apoptosis rates of cells in 100 μg/L (23.78% ) and 200 μ/L TSPG group ( 33.98% ) were higher than those in 10 μg/L TSPG group ( 16. 67 % ), with significant difference( P 〈0. 01 or P 〈 0. 05 ). The expression rates of Survivin were decreased by the treatment with the increasing concentrations of TSPG ( P 〈 0. 05 ). Conclusion TSPG can restrain the human leukemic cell growth and induce cell apoptosis,which may be related to the decreased expressions of Survivin.
出处
《中国基层医药》
CAS
2009年第7期1218-1219,1346,共3页
Chinese Journal of Primary Medicine and Pharmacy
关键词
人参总皂苷
K562细胞
凋亡
生存素
Total saponins of panax ginseng
KS62 cells
Apoptosis
Survivin