人参总皂苷对白血病细胞K562凋亡及生存素表达的影响

Effects of TSPG on apoptosis of K562 cells and the expression of Survivin

目的探讨人参总皂苷（TSPG）对人白血病细胞株K562生长和凋亡的影响及其可能机制。方法采用噻唑兰比色法（Mar）观察TSPG对K562细胞生长的影响；流式细胞仪观察TSPG对细胞凋亡的影响；用RT-PCR检测TSPG诱导K562细胞凋亡中生存素（Survivin）基因的表达情况。结果TSPG对K562细胞生长有抑制作用，呈剂量依赖关系（P〈0．05）；10、100、200μg／ml组细胞凋亡率分别为16．67％、23．78％、33．98％，药物组与对照组差异有统计学意义（P〈0．01或P〈0．05）；随着TSPG浓度升高，Survivin基因的表达水平逐渐下调（P〈0．05）。结论TSPG可以抑制人白血病细胞的生长并诱导其凋亡，其机制可能与下调Survivin基因的表达有关。

Objective To study the effects of TSPG on apoptosis of K562 cells and the probable mechanism involved. Methods MTT was used to investigate the proliferation of K562 cells; Flow cytometry （FCM） was used to investigate the effects of TSPG on apoptosis of K562 cells ; The expression of Survivin in K562 cells treated with different concentraction of TSPG were examined by RT-PCR. Results The growth of K562 cells was inhibited by TSPG in the concentration dependent manner （P 〈 0. 05 ）. FCM showed that the apoptosis rates of cells in 100 μg/L （23.78% ） and 200 μ/L TSPG group （ 33.98% ） were higher than those in 10 μg/L TSPG group （ 16. 67 % ）, with significant difference（ P 〈0. 01 or P 〈 0. 05 ）. The expression rates of Survivin were decreased by the treatment with the increasing concentrations of TSPG （ P 〈 0. 05 ）. Conclusion TSPG can restrain the human leukemic cell growth and induce cell apoptosis,which may be related to the decreased expressions of Survivin.