冠心病患者白介素-8与P-选择素检测的临床意义

Significance of soluble P-selectin and IL-8 in patients with coronary artery disease

目的:探讨血清白介素-8(IL-8)和可溶性P-选择素(Ps)水平变化在监测冠心病的病情及与冠状动脉病变程度的关系。方法:148例老年冠心病患者,按临床诊断分为三组:急性心肌梗死组(AMI)50例、不稳定性心绞痛组(UAP)50例、稳定性心绞痛组(SAP)48例和对照组50例。用酶联免疫吸附试验检测各组血清白介素-8和可溶性P-选择素的水平,并比较各组间的差异。并对冠心病患者的血清白介素-8变化及其与血清可溶性P-选择素水平进行直线相关分析。结果:AMI组、UAP组及SAP组的血清白介素-8和可溶性P-选择素水平比对照组高(P<0.05);AMI组、UAP组白介素-8和可溶性P-选择素水平和SAP组相比,其值增加明显;AMI组和UAP组结果相似;冠心病患者血清可溶性P-选择素水平与白介素-8变化呈正相关。结论:血清可溶性P-选择素和白介素-8可能是老年冠状动脉粥样硬化的标志,参与了冠心病的发病过程,其值与冠状动脉病变程度密切相关。

Objectives： To study the relationship of soluble P-selectin and IL 8 with the severity of coronary artery disease （CAD）, and to investigate whether they are a non-invasive marker of coronaryplaque destabilization in CAD. Methods： The 3 study groups included 50 cases of acute myocardial infarction （AMI group）within 14 hours after the onset, 50 cases of unstable angina （UAP group）within 14 hours after the last episode, 48 cases of stable angina （SAP group） and the control group consisting of 50 cases . Serum level of soluble P-selectin and IL-8 were measured by means of enzyme linked immunosorbent assay. The differences of soluble P-selectin and IL-8 levels between different groups were observed. Pearson correlation analysis test was used to analyze the relationship between the levels of soluble P-selectin and IL-8 in CAD. Results ： The levels of soluble P-selectin and IL-8 were elevated in AMI, UAP and SAP groups as compared with the control group. Compared with SAP group, the levels of soluble P-selectin and IL-8 were elevated in AMI and UAP groups, while there was no significant difference between AMI and UAP groups. The levels of soluble P-selectin and IL- 8 were correlated with the variation of platelet count in peripheral blood of CAD patients. Conclusion： Soluble P-selectin and IL-8 can be used as markers of the presence of coronary atherosclerosis and are involved in the pathogenesis of CAD. Measurement of them may be helpful for diagnosing coronary plaque destabilization and the severity of CAD. The levels of soluble P-selectin and IL-8 correlate with the severity of coronary artery disease （CAD）.