摘要
目的:通过采用不同方法筛查唐氏综合征(DS),以选择利于在条件有限的基层医院开展产前筛查。方法:采用金标法半定量快速筛查,判读结果;采用酶联免疫法检测孕妇血清甲胎蛋白(AFP)和游离β人绒毛膜促性腺激素的含量(Free-β-hCG),进行DS风险率计算。结果:接受筛查的孕妇9554例,其中采用金标法筛查4197例,DS筛查阳性孕妇243例,占筛查总数的5.79%,有47例接受羊水产前诊断,确诊为DS 5例,其他染色体异常7例;采用酶联免疫法筛查5357例,DS筛查为高风险孕妇444例,占筛查总数的8.29%,有207例接受羊水产前诊断,确诊胎儿为DS 8例,其他染色体异常26例。结论:金标法是一种有效的产前筛查方法,可用于一些出生率低、条件有限的中小型医院,对在基层卫生组织广泛开展与普及产前筛查工作有积极作用。
Objective : To evaluate the effectiveness of different methods in screening of Down syndrome, choose preferable method for prenatal screening in primary hospital. Methods: The levels of serum alpha fetoprotein and freeβ- hCG in pregnant women were detected by dot immunogold filtration assay and enzyme linked immunosorbent assay, and the risk ratio was calculated. Results : Among 9 554 cases, 4 197 cases were detected by dot immunogold filtration assay, the positive rate was 5.79% (243 cases) , 47 eases received amniotic fluid examination, and 5 cases were diagnosed as Down syndrome, 7 cases were diagnosed as chromosome abnormal/ties; 5 357 cases were detected by enzyme linked immunosorbent assay, the high risk rate was 8. 29% (444 cases) , 207 eases received amniotic fluid examination, and 8 fetuses were diagnosed as Down syndrome, 26 cases were diagnosed as chromosome abnormalities. Conclusion: Dot immunogold filtration assay is an effective method in prenatal screening of Down syndrome, which can be used in primary hospitals.
出处
《中国妇幼保健》
CAS
北大核心
2009年第23期3321-3323,共3页
Maternal and Child Health Care of China
关键词
唐氏综合征
金标法
酶联免疫法
产前筛查
Down syndrome
Dot immunogold filtration assay
Enzyme linked immunosorbent assay
Prenatal screening