流动注射后化学发光法测定盐酸阿比朵尔

Determination of arbidol hydrochloride by a flow-injection post-chemiluminescence method

研究了盐酸阿比朵尔在鲁米诺-K3Fe（CN）6化学发光反应体系中的后化学发光反应。据此建立了测定盐酸阿比朵尔的流动注射后化学发光分析法。方法的线性范围为6．0×10^-9 ～1．0×10^-7 g／mL，检出限（3σ）为2×10^-9 g/mL。对4．0×10^-8 g／mL的盐酸阿比朵尔溶液连续11次平行测定，RSD=1．3％。方法已用于盐酸阿比朵尔胶囊及尿样中盐酸阿比朵尔含量的测定。在对这一后化学发光反应的动力学性质、化学发光光谱、紫外可见吸收光谱以及一些相关问题研穷的基础上，提出了可能的反应机理。

A new post-chemiluminescence （PCL） phenomenon was observed when arbidol hydrochloride solution was injected into the solution after the finish of CL reaction of alkaline luminol and potassium ferricyanide. A possible reaction mechanism was proposed based on the studies of the CL kinetic characteristics, the CL spectra, and other experiments. Using the PCL reaction, a new method for the determination of arbidol hydrochloride was established. The lin- ear response range of this method was from 6.0× 10^-9 to 1.0 × 10^-7 g/mL with a linear correlation coefficient of 0.9991. The relative standard deviation for 4.0 × 10-8 g/mL solution was 1.3% （n = 11）. This method has been successfully applied to the determination of arbidol hydrochloride in arbidol hydrochloride capsules and urine samples with satisfactory results.