酶催化动力学光度法测定肾上腺素

Determination of adrenaline by enzymatic catalytic spectrophotometry

基于肾上腺素对血红蛋白酶催化体系的抑制作用，建立了酶催化动力学光度法测定肾上腺素的新方法。实验研究了体系的最佳条件及动力学行为，测定的线性范围为4．5×10^-7～1．4×10^-05 mol／L，方法检出限为5．2×10^-08 mol／L。对浓度为9．0×10^-6 mol／L的肾上腺素进行11次平行测定的相对标准偏差为3．5％。此方法可用于药剂中肾上腺素含量的测定。

A high sensitive and simple spectrophotometric method for the determination of adrenaline based on the inh- ibition of hemoglobin for the oxidation of Ha O2 with acid chrome blue K was developed. The percentage inhibition （ 1% ） of system is calculated under the optimal experimental conditions. The calibration curve is linear in the range of 4.5 × 10^-7 - 1.4 × 10-5 mol/L with the detection limit of 5.2 × 10-8 mol/L. The relative standard deviation of this method is 3.5% for 11 determinations of 9.0 × 10-6 mol/L adrenaline. This method can be used for the determination of adrenaline in pharmaceuticals with satisfactory results.