气相色谱-质谱联用检测水中超痕量雌性激素

GL-MS Determination of Ultratrace Amounts of Estrogen

雌性激素对水环境中影响越来越大,因为这些化合物在浓度低至0.1 ng/L时,都会导致雄性鱼的雌雄性别的变化.为了寻找一种具有高灵敏度和高选择性的分析方法用于复杂样品中的雌性激素化合物的检测,研发一种不需衍生化反应的气相色谱-质谱联用直接检测技术来测定水中超痕量的雌性激素(雌激素酮,E1;17β-雌二醇,E2;17α-乙炔基雌二醇,EE2).结果表明:对于雌性激素而言,低温进样口可以有效提高方法的分析灵敏度.优化后的方法具有优良的线性范围,精度及准度,方法检测限分别为0.082 ng/L E1,0.073 ng/L E2,及0.065 ng/L EE2.

Estrogen has greater and greater influence on water environment. Even when the concentration of estrogen is as low as 0.1 ng/L,make fish will reverse sex. In order to find a highly sensitive and selective method to determine estrogen in complex samples,GS-Ms method,which does not need derivative reaction,is developed to determine ultratrace amounts of estrogen in water,including estrone,E1; 17β-estradiol,E2 ; ethinyl estradiol,EE2. The results show that for estrogen,low-temperature injection port can effeciently improve sensitivity of that method. The optimized method has been validated with good linearity,precision and accuracy. Method detection limits （MDL） of each estrogen compounds are found to be 0.082 ng/L for E1,0.073 ng/L for E2,and 0. 065 ng/L for EE2, respectively.