肉苁蓉属植物中六种苯乙醇苷类化合物抗氧化活性的构效关系研究

Structure-activity Relationships of Phenylethanoid Glycosides in Plants of Cistanche salsa on Antioxidative Activity

目的:研究肉苁蓉属植物中苯乙醇苷类化合物抗氧化活性的构效关系。方法:采用DPPH.自由基分析法,对肉苁蓉属植物中的6种苯乙醇苷单体成分进行抗氧化活性的测试,根据其结构特征和活性测定结果,初步推断苯乙醇苷类化合物抗氧化活性的构效关系。结果:不同化学结构的苯乙醇苷化合物清除自由基的能力存在一定差异,其抗氧化能力的顺序为2′-乙酰基毛蕊花糖苷(毛蕊花糖苷≥管花苷B≥异毛蕊花糖苷>松果菊苷>肉苁蓉苷A,它们清除自由基的能力与浓度呈明显量效关系。结论:肉苁蓉属植物中苯乙醇苷类的抗氧化活性与苷元及苯丙烯酰基上的酚羟基数目、酚羟基连接位置、化合物空间位阻的大小、中心葡萄糖2位上乙酰基的取代均存在一定关系,与苯乙烯酰基上的α,β不饱和酮结构片断可能也有一定关联。

Objective ： To study the structure-activity relationships of phenylethanoid glycosides in plants of Cistanche salsa on antioxidative activity, Methods： By the assay systems of DPPH · , the antioxidant activity of six phenylethanoid glycosides from plants of Cistanche salsa was determined to investigate the relationship between the antioxidant activities and phenylethanoid glycosides＇s structural characteristics. Results： The antioxidative activity of phenylethanoid glycosides was variant with dose-dependent effect. The sequence of the strength of the antioxidative activity of the six components was shown to be 2＇-Acetylacteoside 〉 Acteoside ≥ Tubuloside B Isoacteoside 〉 Echinacoside 〉 Cistanoside A. Conclusion： The antioxidative activity of phenylethanoid glycosides is related to the number of phenolic hydroxyl, steric hindrance, 2-acetyl on the middle glucopyranose, and the location of phenolic hydroxyl. Additionally, it may be related to the a, fl-unsaturated ketone of phenl-2-propenoyl.