痰咳净粉雾剂对流感病毒FM1感染小鼠肺部炎性损伤的影响

Effects of Tankejing Dry Powder Inhaler on the Inflammatory Damage in the Lungs of Mice Infected by FM1

目的:探讨痰咳净粉雾剂对流感病毒感染小鼠肺部炎性损伤的影响及作用机理。方法:以流感病毒甲型鼠肺适应株(FM1)滴鼻感染小鼠建立病毒性肺炎模型,随机分为正常对照组、模型组、痰咳净粉雾剂低、中、高剂量组、利巴韦林组。运用Western-Blot检测感染24 h后NF-κB在肺组织中的表达,阐明药物的调控作用;采用ELISA法检测感染3 d后,肺组织炎性细胞因子TNF-α、IL-1β和IL-6水平的变化;同时观察痰咳净粉雾剂不同剂量对小鼠肺指数及病毒致小鼠死亡率的影响。结果:痰咳净中、高剂量有以下作用:①对感染病毒24 h后的小鼠肺组织中NF-κB表达有下调作用;②能降低小鼠肺组织中TNF-α、IL-1β和IL-6水平(P<0.05);③能降低肺指数;④能改善感染小鼠的临床症状,减少15 d内小鼠的死亡数,并能延长其平均存活时间(P<0.05)。结论:痰咳净粉雾剂可通过调节NF-κB以及炎性细胞因子TNF-α、IL-1β和IL-6的表达水平,从而减轻FM1流感病毒引起的小鼠肺部炎性损伤。

Objective：To study the effect of Tankejing Dry Powder inhaler on inflammatory in lung injury of mice infected by influenza virus（ FM1 ）. Methods：Model mice infected by influenza virus FM1 were randomly divided into six groups：the nomal group, model group,low dose, medium dose and high dose of Tankejing Dry Powder inhaler group, and Ribavirin group. The following indices including the change of NF-KB in lung tissue at 24 h, the changes of TNF-α, IL-1β, IL-6 in lung tissue, the lung index and the death rate were observed. Results ： Compared with model group, medium and high dosages of Tankejing Dry Powder inhaler could modulate the expression of NF-κB, reduce the level of TNF-α, IL-1β, IL-6 in lung tissue （P 〈 0. 05 ）, and reduce the lung index and the death number of animals within 14 days （P 〈 0.05 ）. Conclusion：Tankejing Dry Powder inhaler may reduce the inflammatory injury caused by influenza virus FM1 through regulating the expression level of NF-κB and cytokine. Key words Tankejing dry powder inhaler; Influenza virus FM 1 ; Lung index ; NF-κB ; Cytokine