荧光法测定面粉中的核黄素

Fluorimetric Determination of Lactoflavin in Flour

采用分子荧光法测定面粉中痕量核黄素的含量.核黄素浓度在1×10-6～5×10-4 g/L范围内与荧光强度呈良好的线性关系,方法的检出限为4×10-8 g/L;对浓度为1×10-5 g/L的核黄素标准溶液进行11次平行测定,得相对标准偏差为3.4%.方法简便快速、灵敏度高、选择性好,已用于面粉中核黄素含量的测定,回收率为98%～105%,结果令人满意.

A fluorimetric method was proposed for the determination of trace lactoflavin in flour. The lactoflavin concentration within a range of 1×10^-6-5×10^-4g/L has a good linear relation, and the detection limit is 4×10^-8g/L, and the relative standard deviation （RSD） is 3.4% for 1×10^-5g/L lactoflavin solution （n=11）. The method has high sensitivity and selectivity, and has been applied for the detection of lactoflavin in flour, and the recoveries between 98%-105% are obtained with satisfied results.