AgNOR和p53在大肠癌中的表达

Expression of AgNOR and P53 in Colorectal Cancer

目的研究AgNOR和p53在大肠癌中的表达及其与大肠癌发生、发展及预后的关系.方法选用大肠癌组织标本80例及相应的癌旁组织标本40例,采用AgNOR染色技术和免疫组织化学法检测大肠癌组织及癌旁组织中AgNOR计数和p53的表达.结果大肠癌中AgNOR计数显著升高,细胞核内AgNOR计数、颗粒分布及形态特点与大肠癌的分化程度、淋巴结转移有相关性(P<0.05);p53在大肠癌组的阳性表达率为66.3%,在癌旁组的阳性表达率为20.8%.两者之间存在显著性差异(P<0.01);大肠癌组织中p53的阳性表达与大肠癌的分化程度、淋巴结转移有相关性(P<0.05),而与大肠癌的发病年龄、性别无关(P>0.05).结论p53的突变在大肠癌的形成和发展中起了促进作用,AgNOR测定对大肠癌的病理学分级有一定的价值,同时说明AgNOR和p53有可能作为大肠癌发生、发展及预后的肿瘤标志.

Objective To study the expression of P53 and AgNOR in tumor tissues and to investigate theirs value in development and prognosis of colorectal cancer. Methods The expression of AgNOR and p53 in 80 colorectal cancers and 40 para-eancer eolorectal tissues were examined by dying and immunohistoehemical technique.The relation between the expression of AgNOR and P53 in colorectal cancer and the parameters of clinic pathology of colorectal cancer were analyzed. Results The amount of AgNOR is high in the colorectal cancer group. The difference between the pathological grades of colorectal cancer was significant（P〈0,001 ）. The positive rate of P53 in the colorectal cancer group was 66.3% and that in the oara-cancer grouo was 20.8%. The difference between the two groups was significant （P〈0.001）. The expression of AgNOR and P53 were correlated to differentiation and lymph node metastasis（P〈0.05 ）, but unrelated to ages of onset and gender（P〉0.05 ）.Conclusion The mutation of P53 promoted the tumorigenesis and development of colorectal cancers. The examination of AgNOR is valuable in grades of colorectal cancer. AgNOR and P53 would be used as the new tumor markers in tumorigenesis, as well as development and prognosis of colorectal cancer.