超滤膜纯化当归多糖的研究

Purification of Polysaccharide from Angelica sinensis by Ultrafiltration Membrane Technology

目的研究超滤法分离纯化当归多糖组分的工艺。方法采用截留不同分子量的膜对当归多糖提取液进行超滤,并对操作参数进行正交设计,以当归多糖为检测指标优选膜分离工艺条件。结果当归多糖分别用相对分子量为200、100、50、20kD的超滤膜进行分离。超滤前多糖含量30.47%,超滤后200kD以下的多糖含量最高,为65.42%。试验确定超滤操作条件为:加1倍量的水,操作温度35℃,操作压力0.3MPa。结论该工艺简单可行,所得到的当归多糖纯度较高。

Objective To isolate and purify polysaccharide from Angelica sinensis by ultraffltration technology. Methods Extract of polysaccharide of Angelica sinensis was ultrafdtrated with different aperture membrane, the separation parameters on membrane separation efficiency of polysaccharide of Angelica sinensis were optimized by and orthogonal test. Results Polysaccharide of Angelica sinensis was separated with 200, 100, 50, 20 kD membrane. The contents of polysaccharide was 30.47% before ultraffltration. The main component of Angelica sinensis polysaccharide was the fraction with 200 kD （65.42%）. The optimum condition of ultrafiltration was obtained as follow： one times of extract volume, 35 ℃, 0.3 MPa. Conclusion The technology was simple and available, and the content of Angelica sinensis polysaccharide was higher.