去白细胞血液最佳制备工艺建立和质量标准的研究

Establishment of the best technology to prepare blood with the removal of leukocytes and investigation of the quality standards

目的研究去白细胞血液制备最佳方法和工艺流程,建立科学去白细胞血液质量标准。方法分别采用无菌导管连接法、直接过滤法、贮存后过滤法对采集全血进行白细胞过滤,分析过滤工艺方法学差异及过滤后血液相关质量指标。结果无菌导管连接法和直接过滤法可在采集后6 h内过滤和制备各种血液成分,保障了血小板和新鲜冰冻血浆的质量。贮存后过滤法和超过96 h直接过滤法比无菌导管连接法、直接过滤法(血液储存<96 h)红细胞膜脆性差异有统计学意义(P<0.05)。超过96 h的直接过滤法和贮存后过滤法在保存期末白细胞介素(IL)-2和IL-6升高,去白细胞效果有所降低。结论血液采集后短时间内立即过滤制备去白细胞血液优于无菌导管连接法和贮存后过滤法。贮存后过滤法增加了血液污染可能,降低去白细胞效果,应当予以取缔。

Objective To establish the scientific quality standards of blood with the removal of leukoeytesby investigating the best preparing technique and process. Methods Collecting blood were filtrated respectively with leukocyte filter by three methods： connection of sterile catheter, direct filtration and filtration after storage. The differences of these methods were contrasted by analyzing the index about blood quality after filtration. Resuits By the two methods： connection of sterile catheter and direct filtration, all blood components were prepared in 6 hours after collection to ensure quality of thrombocyte and fresh frozen plasma. Compared with the abovementioned, the methods of filtration after storage and direct filtration （the storage time of blood are more than 96 hours） have some limitations： the fragility of erythrocyte membrane was increased, the level of IL-2 and IL-6 were elevated and the effect of removing leukocytes was decreased. Conclusion The method of filtrating collected blood in a short time to prepare blood with the removal of leukocytes is superior to the methods of connection of sterile catheter and filtration after storage. Especially the method of filtration after storage should be abandoned because the possibility of blood contamination was increased and the effect of removing leukocytes was reduced.