黎族脑梗死ApoE基因多态性及饮食因素与血脂相关性研究

Relationship study on ApoE gene polymorphism,serum lipid levels and dietary factors in Li national patients with cerebral infarction

目的:探讨海南黎族脑梗死患者ApoE基因多态性及饮食因素与血脂的关系。方法:对受试者进行血脂检测,采用聚合酶链反应-限制性片段多态性方法检测ApoE基因多态性,并进行饮食因素调查,分析血脂异常与饮食因素及ApoE的关系。结果:共检测出6种基因型,ε4等位基因与TC、LDL-C正相关,ε2则为负相关;其TC、TG水平由高到低依次为嗜油食(嗜甜食(嗜吸烟(嗜饮酒(P(0.05),其中嗜素食、嗜槟榔者TC、TG无统计学意义(P(0.05)。结论:黎族脑梗死患者ApoE基因多态性与血脂水平显著相关,不同的饮食方式对血脂水平有一定影响。

Objective： To investigate the relationship of ApoE gene polymorphism, serum lipid levels and dietary factors in Li national patients with cerebral infarction. Methods： Serum lipid levels were detected in all subjects, ApoE gene polymorphism was tested by polymerase chain reaction and restriction fragment-length poly- morphism （PCR-RFLP）, and their dietary factors were investigated, the relationship of ApoE gene to dyslipidemia and dietary factors were analyzed. Results： 6 genotypes were showed in this study, ε4 allele was positively correlated to TC and LDL-C, while ε2 was negatively correlated, As to diet, the level of TC and TG from high to low was high-fat-diet 〉 sweetaholic 〉 tobaccoholic 〉 alcoholic （P 〈 0.05）, TC and TG had no differences in vegan and betelnut-liker（ P 〈0.05 ）. Conclusions ： ApoE gene polymorphism is correlated to serum lipid levels in Li national patients with cerebral infarction, and different dietary styles also contribute to serum lipid level.