大鼠肝慢性损伤后血浆白蛋白mRNA和丙氨酸转氨酶及羟脯氨酸的变化

Changes of plasma mRNA,alanine aminotransferase and hydroxyproline in chronic liver damage of rats

目的:探讨大鼠肝脏纤维化损伤后血浆白蛋白mRNA水平、丙氨酸转氨酶、羟脯氨酸的活性变化用于评估早期肝损伤的可能性。方法:建立大鼠肝纤维化模型,在不同时间分离血清,采用实时定量多聚酶链反应技术测量血浆中白蛋白的mRNA水平;生化检测血浆丙氨酸转氨酶(ALT)、羟脯氨酸(Hyp)活性。结果:血浆中白蛋白mRNA水平在肝纤维化早期显著增加,丙氨酸转氨酶、羟脯氨酸在肝纤维化早期变化不明显。结论:在大鼠慢性肝损伤早期检测血浆中白蛋白mRNA比丙氨酸转氨酶、羟脯氨酸活性更具敏感性。

Objective： To investigate serum albumin （AST） and Hydroxyproline（Hyp） in injury of rat liver fibrosis. mRNA level, activity of alanine aminotransferase Methods： The rat liver fibrosis model was created by injection Bovine Serum Albumin（BSA）, separated from the serum at different times, using real-time quantitative Polymerase Chain Reaction technique measured the plasma level of albumin mRNA, then detection activity of plasma ALT and Hyp by chemical and biological technique. Results： The plasma level of mRNA was detected a significant increase in the early stage of liver fibrosis, ALT and Hyp were not significantly changed in the early stage of liver fibrosis. Conclusion ： Detection of serum Albumin mRNA has more sensitive activity than plasma Alanine Aminotransferase and Hydroxyproline in the early of chronic liver damage in rats.