HBV Pre-S1蛋白及HBV-DNA含量检测在诊断乙肝病毒复制中的临床意义

Clinical significance of determination of HBV Pre-S1 and HBV-DNA in the identification of HBV replication

目的:探讨乙肝患者不同血清学标志物模式(HBV-M)与乙肝病毒DNA含量及乙肝病毒前S1蛋白的关系,为预测乙型肝炎病毒的传染程度、预防、诊断及治疗提供实验室依据。方法:采用ELISA法对1385例携带不同血清学标志物的乙肝患者进行HBVPre-S1和乙肝5项指标检测,并用聚合酶链反应定量检测血清HBV-DNA含量。结果:不同HBV免疫模式中HBsAg、HBeAg和HBcAb同时阳性,其HBV-DNA和HBVPre-S1的检出率分别为90.7%和71.1%;HBsAg、HBeAb和HBcAb阳性组的检出率分别为35.8%和27.3%;其他不同血清学模式组也有不同的阳性率。结论:HBV-DNA、乙肝病毒5项标志和HBVPre-S1联合检测,对HBV感染早期诊断,了解病毒的复制情况,疗效观察和预后判断均具有重要意义。

Objective： To investigate the relationship between the serological markers of hepatitis B and the quantity of HBV-DNA and HBV Pre-S1, and to provide references for prediction ofinfectivity, prevention and treatment of hepatitis B. Methods： Blood samples of 1 386 patients with hepatitis B were analysed, five serological markers of hepatitis B and HBV- PreS1 was measured with ELISA, and HBV-DNA was determined by real-time quantitative PCR. Results： HbsAg, HbeAg and HbcAb were all positive in different determinations, in which the HBV-DNA and HBV PreS1 positive rates were 90.7% and 71.8% respectively; in patients with HbsAg and HbcAb positive, the HBV-DNA and HBV PreS1 positive rates were 35.8% and 27.3% respectively; , HbeAb the HBV DNA and HBV-PreS1 positive rates varied accordingly in other groups of serological markers. Conclusion： Determining the HBV-DNA,5 serological markers of hepatitis B and HBV-PreS1 simultaneously has great significance for the early diagnosis of HBV infection, monitoring of virus replication, evaluation of therapeutic effects and prognosis.