当归水煎醇沉液对四氯化碳致小鼠肝损伤的保护作用

Protective effects of angelica on tetrachloride-induced liver injury in mice

目的:研究不同剂量的当归水煎醇沉液对四氯化碳致小鼠肝损伤的保护作用。方法:取小鼠50只,随机均分为5组:对照组(Ⅰ)、模型组(Ⅱ)、当归高剂量组(Ⅲ)、当归中剂量组(Ⅳ)、当归低剂量组(Ⅴ)。造模前,Ⅲ、Ⅳ、Ⅴ组小鼠分别按20.0、10.0、5.0g/kg皮下注射当归水煎醇沉液,Ⅰ、Ⅱ组注射等量生理盐水,1次/d,连续6d,末次给药后2h,Ⅱ、Ⅲ、Ⅳ、Ⅴ组小鼠腹腔注射0.1%四氯化碳石蜡油溶液(0.1mL/10g)建立肝损伤模型,Ⅰ组注射等量石蜡油。禁食不禁水,24h后处死小鼠。取肝脏左叶相同部位组织,10%的甲醛固定,制作组织切片,H.E染色,光镜下观察肝组织结构与病理变化。结果:当归水煎醇沉液高、中、低剂量组肝细胞损害程度轻于模型组。结论:当归水煎醇沉液对小鼠四氯化碳致肝损伤有明显的保护作用。

Objective： To study the protective effects of angelica decoction on tetrachloride-induced liver injury in mice. Methods： 50 mice were randomly divided into control group（Ⅰ）,model group（Ⅱ） and high,middle and low dosage of angelica groups（Ⅲ,Ⅳ,Ⅴ）. Groups Ⅲ-Ⅴwere hypodermiclly injected with angelica decoction at 20.0,10.0 and 5.0 g/kg everyday for continuous 6 days,respectively,groupⅠand Ⅱ were given equal dosage of phisiological saline. After the last administration for 2 hours,groups Ⅱ-Ⅴwere abdominally injected with tetrachloride solution at 0.1 mL/10 g to make liver injury model,group Ⅰ was given equal dosage of paraffine. All mice were killed after 24 hours. Slice from left liver samples were fixed by 10 percent formaldehyde and dyed by H.E. Then the liver tissue stucture and pathological change were observed. Results： Liver injury in groups Ⅲ-Ⅴ was mild compared with model group. Conclusion： Angelica decoction could protect liver from tetrachloride-induced injury in mice.