响应曲面法优化中空纤维膜分离啤酒废酵母活性成分的研究

Optimization of the Separation of Waste Beer Barm through Hollow Fiber Membrane by Use of Response Surface Methods

以啤酒废酵母为原料,研究中空纤维膜分离酵母活性成分的工艺。在膜通量试验基础上,选择料液浓度、pH和操作压力为自变量,海藻糖透过率和蛋白质截留率为响应值,采用Box-Benhnken设计的方法,拟合得到二次多项式回归方程的预测模型,并确定膜分离工艺的最佳条件为压力18psi、温度24℃、pH6.0和料液浓度3.0%。在此条件下,膜通量为20.62L/m2·h,海藻糖透过率为96.82%,蛋白质截留率为95.04%。

The separation techniques of active compositions from waste beer barm through hollow fiber membrane were studied. Based on membrane flux experiments, the solution concentration and pH values and operating pressure were selected as independent variables and the penetration rate oftrehalose and the retention rate of proteins selected as response values, then the predictive polynomial quadratic equations model was obtained by use of Box-Benknken design and the optimum separation technical conditions were summed up as follows： operating pressure as 18 psi, temperature at 24 ℃, pH value was 6.0 and solution concentration was 3.0 %. Under the above conditions, the membrane flux was 20.62 L/m^2·h, the penetration rate oftrehalose was 96.82 %, and the retention rate of protein was 95.04 %.