摘要
建立了利用HPLC-ELSD测定木质纤维素原料预处理后水解液中主要糖的成分葡萄糖和木糖含量的方法。实验条件优化得出在漂移管温度为90℃,流动相为乙腈∶水(70∶30),流速为1mL/min,氮气流速为2.0L/min条件下,经Shodex Asahipak NH2P-50(4.6m×250mm,5μm)色谱柱后,可以将水解液中的主要糖成分葡萄糖和木糖进行良好的分离。结果表明,葡萄糖在0.0094～110mg/mL范围内,木糖在0.031～62.34mg/mL内标准曲线相关系数均为0.999,精密度高(以木糖和葡萄糖的相对标准偏差分别为0.54%和0.66%)。该方法具有简洁、快速、准确、重现性好的特点。
The measurement method of main sugar content including glucose and xylose by high-performance liquid chromatography with evaporative light scattering detection （HPLC-ELSD） before and after the treatment of lignocellulose was developed. The optimum technical conditions were obtained as follows： the experimental drift tube at a temperature of 90 ℃, mobile phase of acetonitrile： water=70：30, flow rate as 1 mL/min, nitrogen flow rate as 2.0 L/min, and a good separation of glucose and xylose in the hydrolysate. The results showed that the correlation coefficient of the determination was 0.999 （between 0.0094- 110 mg/mL for glucose and between 0.031 -62.34 mg/mL for xylose）.The method had high precision with RSD as 0.54 % for glucose and 0.6 6 % for xylose respectively.The method had the advantages including simple operation, rapid measurement, high accuracy and good repeatability.
出处
《酿酒科技》
2009年第8期125-127,共3页
Liquor-Making Science & Technology
基金
宜昌市科技攻关项目(A2007103-1)
横向项目"纤维素乙醇的相关关键技术的研究"(200800230)
