报春石斛再生体系的建立

Regeneration system for Dendrobium primulinum

以茎段为外植体,建立了报春石斛Dendrobium primulinum的离体培养再生体系。结果表明:愈合组织诱导以培养基MS(Murashige and Skoog)+5.0mg·L-16-苄氨基嘌呤(6-BA)+1.5mg·L-12,4-D为最优,诱导率达33.33%;分化和增殖培养基均以MS+1.0mg·L-16-BA+0.1mg·L-1萘乙酸(NAA)+体积分数10%椰子水为佳,分化率最高可达83.20%,增殖倍数达3.82,外源物质椰子水可促进不定芽的形成及增殖。形成的无根苗转移到1/2MS+0.5mg·L-16-BA+1.0mg·L-1NAA+0.5g·L-1活性炭培养基上诱导生根发育形成完整植株。

An in vitro plant regeneration system ofDendrobium primulinum was established using stem sections as explants. Results showed that the optimum callus inducing medium was Murashige and Skoog （MS） ＋ 5.0 mg.L^-1 6-BA ＋ 1.5 mg.L^-1 2, 4-D with an induction rate of 33.3%. Adventitious buds were effectively generated and proliferated on MS ＋ 1.0 mg.L^-16-BA ＋ 0.1 mg.L^-1 NAA ＋ coconut water 10% with a differentiation rate of 83.2% and proliferation of 3.82 times. Exogenous coconut water promoted adventitious bud generation and proliferation. The best rooting medium was 1/2MS ＋ 0.5 mg.L^-1 6-BA ＋1.0 mg.L^-1NAA ＋ 0.5 g.L^-1 activated carbon. [Ch, 1 fig. 4 tab. 12 ref.]