安定-氯胺酮对严重烧伤小鼠腹腔巨噬细胞JAB1蛋白表达的影响

Effects of diazepam-ketamine on protein expression of JAB1 in peritoneal macrophages of mice with severe burn injury

目的观察严重烧伤早期小鼠腹腔巨噬细胞c～Jun激活区结合蛋白1（JAB1）蛋白表达变化，探讨安定一氯胺酮对其表达变化的影响。方法将成年健康雄性昆明小鼠随机分为4组，即对照组、严重烧伤组、安定～氯胺酮组和严重烧伤后安定～氧胺酮处理组，以20％～30％体表总面积（TBsA）Ⅲ度烧伤作为严重烧伤模型，肌肉注射安定0．4mg／kg、氯胺酮10mg／kg给予麻醉。收集腹腔巨噬细胞提取总蛋白，运用Westernblotting方法检测各组小鼠腹腔巨噬细胞JAB1蛋白表达水平的变化。结果在严重烧伤后30min，小鼠腹腔巨噬细胞JAB1蛋白表达水平与对照组相比明显降低（P〈0．05），于6h时达到最低（P〈0．01），严重烧伤后12h逐渐恢复至正常水平。然而，严重烧伤后应用安定氯胺酮麻醉，小鼠腹腔巨噬细胞JAB1蛋白表达水平在各时相点变化差异无统计学意义。结论严重烧伤可引起小鼠腹腔巨噬细胞JAB1蛋白表达水平的早期下降，而严重烧伤后应用安定一氯胺酮麻醉能显著抑制JAB1蛋白表达水平下调。

Objective To explore the expression changes of c-Jun activation domain binding protein 1 （JAB1） in mice peritoneal macrophages at early stage of severe burn injury,and to investigate the effects of diazepam ketamine on the expression changes of JAB1 in peitoneal macrophages of mice with severe burn injury. Methods Healthy adult Kunming mice were randomly divided into 4 groups, namely control group, severe burn injury group, diazepam-ketamine anesthesia group,and anesthesia after severe burn injury group. The burn model,used third degree burn of 20%-30% of the total body surface area （TBSA）. Mice in anesthesia after severe burn injury group were anesthetized with diazepam（0.4mg/kg IM） and ketamine （10mg/kg IM） after burn injury. Mice peritoneal macrophages were collected and total protein was extracted. Western blotting assay was used to detect the protein expression level of JAB1 in peritoneal macrophages of every group mice. Results The protein expression level of JAB1 in peritoneal macrophages of mice was markedly declined at 30min after severe burn injury compared with the control group（P〈0.05） ,reached the lowest at 6h（P〈0. 01）, resumed to normal level 12h after severe burn injury. However, the protein expression level of JAB1 did not change significantly in peritoneal macrophages of mice with diazepam-ketamine anesthesia after severe burn injury. Conclusion Severe burn injury can significantly decrease the protein expression level of JAB1 in peritoneal macrophages of mice. However, the down-regulation of the protein expression level of JAB1 could be prevented by diazepam-ketamine anesthesia after severe burn injury.