大豆遗传群体选择与品质QTL的获得

Selection of Genetic Population and Obtaining of the Quality QTLs in Soybean

本文以高油品种哈交97-5404-1为母本,以哈交99-5448-4为父本,建立重组自交系群体。应用SSR技术,对不同世代F2:3、F2:6、F2:9遗传群体中的油份QTL定位进行了分析,不同世代大豆分离遗传群体中油份含量均接近于正态分布,油份含量性状表达偏向于母本哈交97-5404-1,F2:3代获得了对油份贡献率较高的QTL,F2:9代则获多个与油份相关的QTL,大豆F2:3和F2:9代的遗传群体适宜用做品质性状的QTL定位,不同世代定位的油份QTL,均与SSR位点Satt193有关,通过对来自全国不同品种的SSR进行了分析和方差分析证实,Satt193在第一等位变异下(即DNA片段长度为270bp)做为油份的筛选标记具有实用性,而在第三等位变异下(即DNA片段长度为220bp)做为蛋白质材料的筛选标记具有应用性。

In this study, the RIL population was formed from a cross between high oil content cultivar hajiao97- 5404-1 as female parent and Hajiao99-5448-4 as male parent. SSR technology was utilized, genetic maps were constructed and QTL tagging ofoil content trait was also analyzed in three different generations F2：3, F2：6, F2：9. The oil content in the different generations was all close to normal distribution. The expression of the oil content trait in the population had trend to that of the female parent hajiao97-5404-1. We obtained the QTL spaces point from F2：3 population which has high contribution rate about oil content, and multiple QTL spaces points about oil content in F2：9 population. The F2：3 and F2：9 were both suitable for genetic map study for some agronomic traits. The QTL spaces points in different populations were all related with SSR locus Satt193. We did some analysis and variance analysis to the SSR from different nationwide cultivars. On the first equipotential line variation （the DNA fragment length 270 bp）, Satt 193 appeared to be a practical selective marker for oil content. However, the third equipotential line variation （the DNA fragment length 220 bp）, the satt193 was the selective marker for protein materials.