玉米抗甘蔗花叶病毒分子标记与检测技术

Validation and Detection System of Two InDel Markers for Sugarcane Mosaic Virus Resistance in Maize

开发实用分子标记和高效检测技术是开展分子标记辅助育种的基础。本文采用"一致性"抗甘蔗花叶病毒QTL区间的InDel-186标记对BC2F2群体[(掖478×齐319)×掖478]的379个单株进行检测,用In-Del-110标记对重组近交系群体(X178×B73)的183个F8家系进行检测。结果表明:(1)InDel-186标记抗病带型(齐319带型)单株的抗病级别平均为1.27±0.25,而感病带型(掖478带型)单株的抗病级别平均为2.57±0.10,两者差异达到显著水平(F=1.62,P<0.02);(2)InDel-110标记抗病带型(X178带型)家系的病株率平均为55.39±7.24,而感病带型(B73带型)家系的病株率平均为81.10±4.89,两者差异达到显著水平(F=0.62,P<0.03);(3)进一步优化PCR反应体系,建立了InDel-186和InDel-1105μL的二重PCR检测体系。采用In-Del-186和InDel-110标记及优化的检测体系可以有效地选择玉米抗甘蔗花叶病毒基因型,提高选择效率。

Development of effective markers and efficient detection system is the basis of marker-assisted breeding program. The validation of two markers lnDel-186 and InDel-110 for sugarcane mosaic virus （SCMV） resistance, developed in conserved domains of disease-resistant genes within the consensus SCMV-resistance QTL on the maize bins 3.04-3.05 and 6.00-6.01, was investigated in 379 individuals of [（Ye478 ×Hai9-21）×Ye478] BC2F2 population and 183 lines of X178×B73 recombined inbred line （RIL） F8 population evaluated under field condition with artificial inoculation, respectively. The average leaf severity scale of resistant individuals （Qi319 profile） amplified by InDel-186 was 1.27±0.25, significantly lower than 2.57±0.10 of susceptible individuals （Ye478 profile） from the BC2F2 population （F=1.62, P〈0.02）. The disease percentage of resistant RILs （X178 profile） amplified by lnDel-110 was 55.39±7.24, significantly lower than 81.10±4.89 of susceptible RILs （B73 profile） derived from the X178×B73 population （F=0.62, P〈0.03）. Furthermore, the PCR amplification procedure was optimized for two markers InDel-186 and InDel-110. It was suggested that the maize breeding materials with SCMV resistance would be effectively developed by marker-assisted selection with InDel-186 and InDel-110.