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甜菜夜蛾几丁质合成酶基因A和B启动子的克隆 被引量：1

Cloning of chitin synthase gene A and B promoters from Spodoptera exigua(Lepidoptera:Noctuidae)
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摘要 几丁质不仅是昆虫的表皮和围食膜的主要成分,也是一个非常关键的害虫控制靶标,主要通过几丁质合成酶(chitin synthase,CHS)基因合成。本文在克隆甜菜夜蛾Spodoptera exigua的两个几丁质合成酶基因(SeCHSA和SeCHSB)cDNA和基因组序列的基础上,从基因的5′末端设计特异性引物和构建特定的基因组文库,采用PCR的方法获得了5′端侧翼序列。通过5′RACE的方法确定SeCHSA和SeCHSB基因的转录起始位点后,获到了启动子序列。这为研究昆虫几丁质合成和转录调控奠定了基础。 Chitin, synthesized by chitin synthase （CHS）, is not only the essential component for epidermis and peritrophic membrane of the midgut in insects, but also a very pivotal target for insect pest control. Based on the cDNA sequences and genomic sequences of the two CHS genes in Spodoptera exigua, the 5＇ flanking sequences were cloned using specific primers. Promoter sequences of SeCHSA and SeCHSB were obtained after initiation sites of transcriptional regulation were verified by means of 5＇ RACE. These results are important for the research of chitin synthesis and transcriptional regulation in insects.
作者 唐斌 王世贵 张文庆
机构地区 杭州师范大学杭州市动物科学与技术重点实验室 中山大学有害生物控制与资源利用国家重点实验室
出处 《昆虫学报》 CAS CSCD 北大核心 2009年第7期736-742,共7页 Acta Entomologica Sinica
基金 "973"计划项目子课题(2006CB102001) 杭州师范大学高层次人才引进科研启动项目(YS05203105)
关键词 甜菜夜蛾 几丁质 几丁质合成酶 启动子 克隆 Spodoptera exigua chitin chitin synthase promoter cloning
分类号 Q966 [生物学—昆虫学]
  • 相关文献

参考文献23

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共引文献36

同被引文献22

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昆虫学报
2009年 第7期

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