上皮性卵巢癌AIB1蛋白表达水平及与雌、孕激素受体表达的相关性研究

Correlation of expression of AIB1 protein with ER and PR in epithelial ovarian cancer

目的检测AIB1蛋白在上皮性卵巢癌中的表达情况,及其表达与雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)表达之间的关系,初步探讨AIB1蛋白在上皮性卵巢癌发生、发展中的作用。方法采用免疫组化S-P法检测正常卵巢及卵巢良、恶性肿瘤组织中AIB1蛋白的表达水平,并分析其与临床病理特征的关系,同时检测卵巢癌中ER、PR的表达情况,并分析其与AIB1表达之间的相关性。结果(1)卵巢癌中AIB1蛋白的阳性表达率为65.2%,明显高于正常卵巢(8.3%)和卵巢良性肿瘤(25.0%)。(2)临床～期的晚期病例中AIB1的阳性表达明显高于～期的早期病例(P=0.000);低分化组较之高、中分化组,有更高水平AIB1蛋白的阳性表达(P=0.022,P=0.018);淋巴结转移组中AIB1的表达明显高于阴性组(P=0.007),未发现不同病理类型间AIB1蛋白表达存在差异。(3)69例卵巢癌中,ER、PR的阳性表达率分别为31.9%、26.1%,未发现AIB1蛋白的表达与ER、PR的表达间存在相关性。结论AIB1可能通过非激素依赖的途径参与了上皮性卵巢癌的发生、发展过程。

Objective To investigate the correlation of expression of AIB1 （amplified in breast cancer 1） protein with estrogen receptor （ER） and progesterone receptor （PR） in epithelial ovarian cancer. Methods Tissue samples were collected from 24 cases of benign ovarian tumors, 69 of ovarian cancer and 24 of normal ovarian tissue. AIB1 expression and ER,PR expression were examined by immunohistochemical S-P method. Results The positive expression rate of AIB1 in ovarian cancers was 65.2% which was significantly higher than that in normal ovarian tissues （8. 3%） and benign ovarian tumors （25. 0%）. The expression of AIB1 protein in stage Ⅲ-Ⅳ was higher than that in stage Ⅰ- Ⅱ （P = 0. 000）. Poorly- differentiated cancers showed higher expression rate compared with well-differentiated and moderately-differentiated cancers （P=0. 022, P= 0. 018）. The correlation of AIB1 protein expression with lymph node involvement was significant （P = 0. 007）. There was no significant difference in AIB1 expression among different histological types. The positive rates of ER and PR in ovarian cancers were 31.9% and 26.1% ,respectively,which was not correlated with AIB1 expression. Conclusion The overexpression of AIB1 may play a role in the carcinogenesis and development of epithelial ovarian cancers through a pathway independent to hormone.