高脂饮食和追赶生长对大鼠胃排空及血浆胰高血糖素样肽-1浓度的影响

The effect of high-fat diet and catch-up growth on gastric emptying and plasma glucagon-like peptide-1 concentration in rats

目的探讨高脂饮食和追赶生长对大鼠胃排空及胰高血糖素样肽-1（GLP-1）浓度的影响。方法sD大鼠60只随机分为4组，即普通饮食组（NC组）、普通饮食追赶生长组（RN组）、高脂饮食组（HF组）和高脂饮食追赶生长组（RH组）；观察大鼠体重和进食量的变化，并分别于实验4、6、8周处死部分动物取材，检测胃排空率及血浆GLP-1浓度。结果与NC组相比，4、6、8周时HF组胃排空率均加快（95．61％比96．16％，94．24％比96．11％，93．08％比95．50％）、GLP-1分泌增加（12．02pmol／L比16．27pmol／L，10．77pmol／L比13．93pmol／L，9．47pmol／L比11．53pmol／L）。与NC组相比，RN组胃排空率4周时明显降低（95．61％比87．60％），GLP-1分泌减少（12．02pmol／L比9．69pmol／L）；6、8周时胃排空率明显加快（94．24％比96．11％，93．08％比94．52％），GLP-1分泌增多（10．77pmol／L比12．57pmol／L，9．47pmol／L比9．80pmol／L）。与HF组相比，RH组胃排空率4周时也明显降低（96．16％比90．70％），GLP-1分泌减少（16．27pmol／L比9．69pmol／L）；6周时胃排空率增加（96．11％比98．02％），GLP-1分泌虽然增多，但仍低于HF组（13．93pmol／L比13．44pmol／L）；8周时胃排空率又趋于下降（96．50％比92．57％），GLP-1分泌仍低于HF组（11．53pmol／L比10．00pmol／L）。结论虽然高脂饮食和追赶生长均影响胃肠道功能，但是追赶生长对胃肠道功能的影响更大，这可能与追赶生长所导致的病理生理变化有关。

Objective To investigate the effect of high-fat diet and catch-up growth on gastric emptying and plasma glucagon-like peptide-1 （GLP-1） concentration in rats. Methods Sixty SD rats were randomly divided into four groups, i.e. normal control group （ NC group）, refed with normal diet group （RN group）, high fat diet group（HF group） and refed with high fat diet group（RH group）. The weight and diet of rats were recorded. At the onset of refeeding （4 weeks ） and after 2 or 4 weeks of refeeding （6 or 8 weeks） , animals were sacrificed and then gastric emptying study test and the assessment of plasma GLP-1 concentration were carried out. Results Compared with NC group, rats in HF group possessed accelerated gastric emptying rate （95.61% vs 96. 16%,94.24% vs 96. 11%,93.08% vs 95.50%） and increased GLP-1 concentration （12. 02 pmol/L vs 16. 27 pmol/L, 10. 77 pmol/L vs 13.93 pmol/L,9.47 pmol/L vs 11.53 pmol/L） at 4,6,8 weeks respectively. Similarly, compared with NC group, RN group possessed slowed gastric emptying rate （95.61% vs 87.60% ） and decreased GLP-1 concentration（ 12. 02 pmol/L vs 9.69 pmol/L） at 4 weeks, but accelerated gastric emptying rate （94.24% vs 96. 11%, 93.08% vs 94. 52% ） and increased GLP-1 concentration （ 10. 77 pmol/L vs 12. 57 pmol/L, 9. 47 pmol/L vs 9. 80 pmol/L） at 6 and 8 weeks respectively. Compared with HF group, RH group obtained lowed gastric emptying rate（96. 16% vs 90. 70% ）and decreased GLP-1 concentration（ 16. 27 pmol/L vs 9. 69 pmol/L） at 4 weeks, hut accelerated gastric emptying rate（96. 11% vs 98.02% ）and slightly lower GLP-1 concentration （13. 93 pmol/L vs 13.44 pmol/L）at 6 weeks , and further slowed gastric emptying rate （96. 50% vs 92. 57 % ）and lower GLP-1 concentration （11.53 pmol/L vs 10. 00 pmol/L）. Conclusion Although both high-fat diet and catch-up growth affect the gastrointestinal function, the latter has a greater impact, which could account for some pathophysiological changes caused by catch-up growth.