两种不同终止子在转基因鲤鱼中的促生长效应

The growth promoting activity of two different kinds of reconstructed terminators in transgenic common carp

转基因构建体中启动子的选择会直接影响转植基因的活性,近年来有研究表明转基因构建体中终止子的选择会一定程度地影响转植基因的活性。为了更好地筛选转基因构建体和培育快速生长的转"全鱼"生长激素(Growth hormone,GH)基因鱼,文章用鲤鱼β-actin基因终止子和生长激素基因终止子分别构建了转基因构建体,显微注射得到转"全鱼"GH基因鱼P0代养殖群体,比较两种不同终止子构建体的活性。统计分析发现,生长激素基因终止子构建体的养殖群体的体重频率呈正态分布且平均体重显著高于β-actin基因终止子构建体的养殖群体,β-actin基因终止子构建体的养殖群体的体重频率呈右倾趋势的非正态分布。值得一提的是在混合养殖组中得到一条生长最为快速的鲤鱼证实为转基因阳性且为生长激素基因终止子构建体的转基因鲤鱼。该结果表明转"全鱼"生长激素基因鲤鱼可快速生长,并能将转植基因向下代遗传。实验结果提示生长激素基因终止子构建体比β-actin基因终止子构建体表现的促生长活性要强。

The transgenic promoter directly affects the activity of transgenic gene. Choice of terminator has been proved to affect the activity of transgenic gene. Since the relevant theoretical research is rare, using the terminator of transgenic gene or the promoter gene to construct the transgenic vector has not come to the conclusion. In order to construct a better transgenic vector to nurture ＂all fish＂ growth hormone transgenic fish which has a rapid growth character, two different transgenic vectors were constructed separately by the terminator of β-actin gene and the terminator of growth hormone gene After micro-injection, we obtained the P0 generation of ＂all fish＂ transgenic fish farming groups. Comparing the activities of the two different transgenic vectors, the weight distribution of the breeding groups from growth hormone gene terminator transgenic vector felt into a normal distribution; however, the weight distribution of the breeding groups originated from β-actin gene terminator exhibited a non-normal mode and in a rightist trend. The average weight of GH （Growth hormone） gene terminator transgenic groups was significantly higher than that of the fl-actin gene terminator transgenic groups. Interestingly, a fast-growth transgenic carp was found from a mixed farming group, which was positive for the growth hormone gene terminator. This result showed that ＂all fish＂ GH gene transgenic carp could grow fast, and the transgenic gene could inherit from generation to generation. Our results suggest that the growth hormone gene terminator transgenic vector has stronger growth-promoting activity than the β-actin gene terminator transgenic vector.