免疫重建荷人高转移肝癌SCID鼠模型的建立及其鉴定

Establishment and identification of HU-PBL-SCID mice model of human high metastatic hepatocelluar carcinoma

目的:建立具有人免疫学特性的高转移肝癌SCID鼠模型。方法:SCID小鼠腹腔注射人外周血淋巴细胞,皮下接种人高转移肝癌细胞MHCC97-H,免疫重建人高转移肝癌SCID小鼠模型,并鉴定建立的结果。结果:①免疫重建荷人高转移肝癌细胞小鼠的成瘤率为100%,较荷瘤组成瘤潜伏期延长,体积缩小(P<0.05);转移率为100%,其从皮下转移到肝脏所需时间较荷瘤组延长(P<0.05)。②第2、4、6周小鼠血中人IgG含量的测定:同期相比,人化荷瘤组小鼠血中人IgG含量高于人化组(P<0.05)。③第6周SCID小鼠外周血中人CD3+T淋巴细胞和人CD20+B淋巴细胞含量的测定:人化荷瘤组小鼠血中人CD3+T淋巴细胞和人CD20+B淋巴细胞含量高于人化组(P<0.05)。④免疫组化检测人化荷瘤组小鼠脾脏中存在人CD3+T淋巴细胞和CD20+B淋巴细胞。结论:成功建立免疫重建荷人高转移肝癌SCID鼠模型,为肝癌转移的研究及治疗提供了理想的动物模型。

Objective： To explore the effective methods of establishing a human metastatic hepatocelluar carcinoma model in human peripheral blood lymphocyte （hu - PBL） engrafted to severe combined immunodeficient （SCID） mice. Methods： The immunological features of mice were evaluated after intra - peritoneal injection of hu - PBL and subcutaneous implantation of human high metastatic hepatocelluar carcinoma cells （ MHCC97 - Hs）. Results：①Subcutaneous tumors developed in all the mice given hu - PBL and MHCC97 - Hs. The latency period was significantly prolonged, and the tumor size was marked depressed, as compared with mice given human MHCC97 - Hs alone. The tumor＇s metastatic time needed of the mice given hu - PBL and MHCC97 - Hs was longer than the mice given human MHCC97 - Hs alone. ②In the 2^nd ,4^th and 6^th week,the amount of human IgG in the mice given hu - PBL and MH- CC97 - Hs was lager than the mice given MHCC97 - Hs alone. ③In the 6^th week,the number of human CD3^＋ T lymphocytes and CD20^＋ B lymphocytes in the mice given hu - PBL and MHCC97 - Hs was lager than the mice MHCC97 -Hs alone. ④ Immunohistochemical staining revealed presence of remarkable human CD3^＋ T lymphocytes and CD20^＋ B lymphocytes in SCID mice spleen. Conclusion： A human high metastatic hepatocelluar carcinoma model has been established hu - PBL engrafted to SCID mice ,which is an ideal animal model for preclinical research and treatment for metastatic of hepatocelluar carcinoma.