肝癌细胞中PI3K信号通路对VEGF表达的影响

The influence on VEGF expression by phosphatidylinositol 3-kinase signaling pathway in hepatocellular carcinoma cells

目的:研究磷脂酰肌醇3-激酶(PI3K)信号通路在肝癌细胞血管内皮生长因子(VEGF)表达调控中的作用。方法:体外培养高转移性人肝癌细胞株HCCLM3,分为对照组、PI3K特异性抑制剂LY294002浓度5μmol/L组,LY294002浓度10μmol/L及20μmol/L组,处理6小时后,采用逆转录聚合酶链反应(RT-PCR)技术检测VEGF mRNA表达的变化。结果:LY294002可使肝癌细胞HCCLM3的VEGF mRNA的表达下降,且这种抑制作用随着LY294002浓度增大而增大(P<0.01),呈现剂量移赖性关系。结论:LY294002可以抑制肝癌细胞株HCCLM3中VEGF的表达,肝癌细胞VEGF的表达调控受PI3K信号通路调控。

Objective： To investigate the roles of phosphatidylinositol 3 - kinase （ PI3 K） in the regulation of expression of vascular endothelial growth factor（VEGF） in hepatocellular carcinoma（HCC） cells. Methods： The high metastasic cell line HCCLM3 were cultured in DMEM medium, HCCLM3 cells were divided into control groups and LY294002 groups （ 5 μM, 10 μM,20 μM ）, LY294002 was the specific inhibitor of PI3 K. The expression of VEGF mRNA was detected by reverse transcriptional polymerase chain reaction （ RT - PCR） after 6 hours. Results： In HC- CLM3 cells, the expression of VEGF mRNA can be apparently inhibited by LY294002. With the increase of LY294002 concentration,the expression of VEGF mRNA decreased gradually（P 〈0.01 ）and in a dose -dependent manner. Conclusion： LY294002 can reduce the expression of VEGF mRNA in HCCLM3 cell line, the expression of VEGF in HCC cells is regulated by PI3K.