摘要
目的:探讨TIMP-3甲基化调控与大肠癌发生发展的关系。方法:随机收集45例大肠癌病例、42例大肠腺瘤病例、48例非肿瘤性大肠病例标本,用甲基化特异性PCR(methylation-specific PCR,MSP)分别检测TIMP-3基因启动子5'CpG岛甲基化状况。结果:非肿瘤性大肠病变、大肠腺瘤、大肠癌分别有0例(0.00%)、5例(11.90%)、14例(31.11%)TIMP-3基因启动子发生甲基化。大肠癌组织TIMP-3甲基化率高于非肿瘤性大肠病变(P<0.01)和大肠腺瘤(P<0.05)。大肠腺瘤TIMP-3甲基化率高于非肿瘤性大肠病变(P<0.05)。大肠癌肝转移组、淋巴结转移组TIMP-3基因甲基化率分别高于非肝转移组(P<0.01)、非淋巴结转移组(P<0.01);Duck'sC+D组甲基化率分别高于Duck'sA+B组(P<0.01)。结论:大肠癌TIMP-3基因的高甲基化在大肠癌的发生、发展中发挥重要作用,对于大肠癌的早期预防、诊断、恶性程度及预后判断有重要意义。
Objective: To explore whether aberrant methylation in the promoter of TIMP - 3 gene was associated with carcinogenesis and clinicopathological characteristics of colon cancer. Methods: Forty five colon cancer specimens ,48 specimens of no tumor disease in colon ,42 adenoma specimens were collected at random. The 5'CpG islands ' methylation status of TIMP - 3 gene were detected by methylation - specific PCR (MSP). Results: The status of CpG islands methylation of TIMP - 3 gene promoter region were higher in colon cancer group than the group of no tumor disease in colon ( P 〈 0.01 ) and adenoma group ( P 〈 0.05 ), were higher in adenoma group than the group of no tumor disease in colon (P 〈 0.05 ). The status of CpG islands methylation of 'FIMP -3 gene promoter region were higher in the groups of lymph node metastasis ,hepatic metastasis and Duck's C + D than in the groups no lymph node metasta- sis, no hepatic metastasis and Duck's A + B (P 〈 0.01 ). Conclusion :The status of CpG islands methylation of TIMP -3 gene promoter region play important role in carcinogenesis and development of colon cancer, It is valuble in the early preventment,diagnosis and prognosis of colon cancer.
出处
《现代肿瘤医学》
CAS
2009年第8期1530-1532,共3页
Journal of Modern Oncology