三七胶囊对大鼠应激性溃疡血管内皮生长因子表达的影响

Effects of Sanqi capsule on the expression of vascular endothelial growth factor in stress gastric ulcer rats

目的:观察三七胶囊对大鼠应激性溃疡血管内皮生长因子(VEGF)表达的影响。方法:采用乙酸灼烧法制作大鼠应激性胃溃疡模型,在不同时间点取大鼠胃溃疡组织,用Western blot印迹技术和RT-PCR技术分别检测VEGF蛋白及基因表达。结果:三七胶囊治疗应激性溃疡1周和4周后其胃黏膜VEGF蛋白及基因表达较雷尼替丁治疗组、模型组、假手术组及正常对照组相比均明显增加(P<0.05)。结论:三七胶囊能够增加大鼠应激性溃疡VEGF表达,可能是其治疗应激性溃疡的机制之一。

Objective：To study the effects of Sanqi capsule on the expression of vascular endothelial growth factor（VEGF） in the acetic acid- induced ulcer of rats. Methods： A gastric ulcer rat model induced by acetic acid was established. After administration of Sanqi capsule, the lesion tissues were obtained at different times, and the expressions of VEGF mRNA and protein were detected by RT - PCR and Western blot methods. Results： Compared with normal, model, pseudo - operated and Ranitidine groups, the expression levels of the VEGF mRNA and protein in gastric mucosa were all up - regulated significantly one and four weeks after treatment in Sanqi capsule group （P 〈 0.05 ）. Conclusion： The VEGF expression in the acetic acid - induced ulcer of rats can be increased by Sanqi capsule, which may be one of the mechanisms involved in the treatment of stress ulcer.