摘要
Background Trichosporon asahfi （T. asahii） is one of the most important pathogenic fungus in the genus of trichosporon. Although the species identification of T. asahfi was based upon the complicated results of morphologic, biochemical and biologic examination, the morphology characteristic is still the first clue to the species. Some common structures of T. asahfi had been described such as arthrofilaments and arthroconidia, but other important structures of T. asahii were unclear. Methods Six strains of T. asahiiwere incubated on the slant and micro culture of Sabouraud＇s dextrose agar at 30℃ for 7 days. Samples were fixed using 2% paraformaldehyde and 2.5% glutaraldehyde. T. asahfi was observed under scanning electron microscope and transmission electron microscope. Results The detailed characteristics of the diverse sites of germination, as well as some uncommon structures such as giant cell, sarcinate, and club-shaped macroconidia, were presented. The pseudohyphae of T. asahii were noted to produce true hyphae, either along the longitude axis or on the flank. T. asahii was noted to have blastic and thallic conidiation. Digitated branches, trichoid structures and septa inside the spores were detected. Conclusion These results may add our knowledge to the structure and development of T. asahii.
Background Trichosporon asahfi （T. asahii） is one of the most important pathogenic fungus in the genus of trichosporon. Although the species identification of T. asahfi was based upon the complicated results of morphologic, biochemical and biologic examination, the morphology characteristic is still the first clue to the species. Some common structures of T. asahfi had been described such as arthrofilaments and arthroconidia, but other important structures of T. asahii were unclear. Methods Six strains of T. asahiiwere incubated on the slant and micro culture of Sabouraud＇s dextrose agar at 30℃ for 7 days. Samples were fixed using 2% paraformaldehyde and 2.5% glutaraldehyde. T. asahfi was observed under scanning electron microscope and transmission electron microscope. Results The detailed characteristics of the diverse sites of germination, as well as some uncommon structures such as giant cell, sarcinate, and club-shaped macroconidia, were presented. The pseudohyphae of T. asahii were noted to produce true hyphae, either along the longitude axis or on the flank. T. asahii was noted to have blastic and thallic conidiation. Digitated branches, trichoid structures and septa inside the spores were detected. Conclusion These results may add our knowledge to the structure and development of T. asahii.
基金
Thiswork was supported by grants from the Eleventh-Five Plan of the People＇s Science Foundation of China （No. 30570099）.Acknowledgements： We thank Prof. WANG Duan-li and Prof. LI Ruo-yu （Department of Dermatology/Research Center of Medical Mycology, Peking University First Hospital） for technical instruction.
