花叶姜离体再生体系的建立

In vitro Culture and Plant Regeneration of Alpinia zerumbet cv.Variegata

以花叶姜茎块为外植体进行离体培养与植株再生研究。结果表明:花叶姜的启动培养基以改良MS+BA 2.0 mg/L+NAA 0.1 mg/L较适宜,继代增殖以改良MS+BA 1.5 mg/L+NAA 0.2 mg/L为宜,增殖系数可达3.8,生根培养基以1/2MS+NAA 0.1 mg/L+IBA 0.1 mg/L为适,生根率达96%,试管苗移栽成活率达97%以上。

Using Alpinia zerumbet cv. Variegata tuber as explants, in vitro culture and rapid propagation were stucued. The results showed that the optimum medium for bud regeneration was MS＋BA 2. 0 mg/L＋NAA 0. 1 mg/L, the optimum subculture medium was MS＋BA 1.5 mg/L＋NAA 0. 2 mg/L, on which each explant produced up 3. 8 plantlets on the average. The frequency of rooting was over 96% on 1/2MS medium supplemented with 0. 1 mg/L NAA and 0. 1 mg/L IBA. The survive rate after trartsplant reached above 97%.