雌激素受体β对牙周膜成纤维细胞OPG表达的影响

Effect of estrogen receptor beta on the expression of osteoprotegerin in human periodontal ligament cells

目的:研究雌激素受体β(ERβ)对人牙周膜成纤维细胞(HPLF)的骨保护因子(OPG)表达的影响。方法:合成ERβ基因的干涉载体,以脂质体法转染至HPLF中,Western blot及RT-PCR法检测转染效率。鉴定后用雌激素干预ERβ干涉载体转染和未经转染的HPLF,研究细胞OPG的表达情况。结果:ERβ干涉载体可特异地抑制HPLF中ERβ的表达。雌激素明显增强了未转染的HPLF的OPG表达,但对稳定转染的HPLF的OPG表达没有显著影响。结论:雌激素主要通过ERβ促进人牙周膜成纤维细胞OPG的表达。

Objective：To investigate the effect of ERβ on the expression of OPG in human periodontal ligament （HPLF）cells. Method： We employed a short interfering RNA （siRNA） technique to inhibit ERβ expression in HPLF cells and cells were cultured with saturating concentration of 17β-estradiol （10^-7 mol/L） for 48 hours. Changes of the expression of OPG were determined by RT-PCR and Western blot analysis. Result： It was shown that estradiol caused an increase in OPG expression in HPLF cells. However, estradiol had no effect on the expression of OPG in HPLF -siERβ cells. Conelusion：These results indicate that ERβ, is of major importance for estrogen-mediated effects on gene transcription in HPLF cells and estrogen influences the expression of OPG in HPLF cells via ERβ.