以胶原膜为支架构建组织工程化人口腔黏膜研究

Primary study of tissue-engineered oral mucosa with scaffold of collagen membrane

目的:应用组织工程技术,以胶原膜为支架构建组织工程化人口腔黏膜。方法:体外培养、扩增人口腔黏膜角化细胞,通过细胞形态学及角蛋白免疫组化染色等对细胞进行定性研究,角化细胞接种于胶原膜上液面下培养1周后,升至液-气平面培养1周,光镜、电镜下观察大体组织形态及超微结构。结果:口腔黏膜上皮细胞可在生物支架材料胶原膜上粘附生长,并可形成上皮样组织,但无成熟的桥粒及基底膜样结构。胶原膜降解快,14d左右难以操作。结论:胶原膜与口腔黏膜角化细胞具有良好的生物相容性;由于降解速度和不易操作等特点,胶原膜作为支架材料构建组织工程化口腔黏膜有待深入研究。

Objective： To reconstruct tissue engineered oral mucosa with scaffold of collagen membranes. Method： Oral keratinocytes were harvested, isolated, and cultured. They were seeded onto collagen membranes for one week culture in liquid and one week air-liquid interface culture. Histomorphology and ultra-structure were observed. Result： Oral keratinocytes could adhere to and proliferate on collagen membranes, and could form epithelia without hemidesmosome and basal membrane; collagen membranes degenerated at day 14 and was difficult to handle. Conclusion： Collagen membrane has good bio-compatibility with oral keratinoeytes; For its characterization of degenerating and handling, further research required to use collagen membrane as scaffold in oral mucosa tissue engineering.