摘要
目的:研究升麻Cimicifuga foetida根茎的化学成分和所得化合物的抑制破骨细胞活性。方法:采用正、反相硅胶等色谱方法进行化学成分的分离纯化,根据谱学数据结合理化性质鉴定化合物结构。并进行了抑制破骨细胞活性研究。结果:从升麻根茎的石油醚和醋酸乙酯提取物中共分得13个环菠萝蜜烷三萜成分,其结构分别鉴定为25-O-乙酰基-升麻醇(1),升麻醇(2),升麻酮醇(3),26-去羟基阿科特素(4),类叶升麻苷A(5),23-表-26-去羟基阿科特素(6),阿科特素(7),12β-羟基升麻醇(8),升麻苷E(9),(23R,24S)25-O-乙酰基-升麻醇-3-O-β-D吡喃木糖苷(10),(23R,24S)升麻醇-3-O-β-D-吡喃木糖苷(11),升麻苷H-1(12)和升麻苷H-2(13)。结论:化合物5为首次从该属植物中分离得到,化合物7为首次从该植物中分离得到,化合物2,7,10,11具有抑制破骨细胞活性。
Objective: To study cycloartane triterpenoid of Cimicifugafoetida and their osteoclast inhibition activity. Method: The compounds were isolated and purified by normal and reversed phase column chromatographic methods. Structures were identified by spectroscopic analyses. Their osteoelast inhibition activity was then studied. Result: Thirteen cycloartane triterpenoids were isolated from the rhizomes of C. foetida and were characterized as 25-O-acety1-cimigenol ( 1 ), cimigenol ( 2 ), cimicidanol ( 3 ), 26-deoxyactein (4), asiaticoside A (5), 23-epi-26-deoxyactein (6), actein (7), 12β-hydroxycimigenol (8), cimiside E (9), (23R, 24S) 25-O-acetyl-cimigenol-3-O-fl-D-xylopyranoside ( 10 ), (23R, 24S) cimigenol-3-O-β-D-xylopyranoside ( 11 ), cimicifugoside H-1 ( 12 ) and cimicifugoside H-2 (13). Conclusion: Compound 5 was isolated from this genus for the first time, compound 7 was isolated firstly from the plant and compound 2, 7, 10 and 11 exhibit osteoclast inhibition activitv.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第15期1930-1934,共5页
China Journal of Chinese Materia Medica
关键词
升麻
环菠萝蜜烷三萜
抑制破骨细胞活性
Cimicifuga foetida
cycloartane triterpenoid
osteoclast inhibiion activity