大鼠心肌组织中布比卡因的LC-MS/MS测定

Determination of Bupivacaine in Rat Myocardium by LC-MS/MS

建立了液相色谱-串联质谱法测定大鼠心肌组织中的布比卡因。采用C18色谱柱,甲醇-0.1%甲酸溶液(55:45)为流动相,利多卡因为内标。采用正离子电喷雾离子源,多离子反应监测(MRM)方式,监测质核比m/z289→140(布比卡因)和m/z285→86(内标)。布比卡因在0.01～20μg/g浓度范围内线性关系良好,萃取回收率为66.4%～74.57%,日内和日间RSD均小于7%。

A LC-MS/MS method was established for the determination of bupivacaine in rat myocardium. A C18 column was used with the mobile phase of methanol-0.1% formic acid solution （55 ： 45） and lidocaine as the internal standard. Electrospray ionization （ESI） source was operated in positive ion mode and multiple-reaction monitoring （MRM） mode. The detected ions were m/z 289→140 for bupivacaine and m/z 285→86 for the internal standard. The calibration curve for bupivacaine was linear in the range of 0.01-20.0 ug/g in myocardium. The recovery was 66.45%- 74.47 % with the intra- and inter-day RSDs less than 7 %.