硝苯地平和机械力对人牙周膜成纤维细胞基质金属蛋白酶的表达影响

The effect of Nifedipine and mechanical strain on matrix metalloproteinase expression in human periodontal ligament fibroblast

目的研究机械静态拉伸作用下,硝苯地平对人牙周膜成纤维细胞(HPDLF)基质金属蛋白酶(MMP)-10和MMP-13的表达影响。方法按弹力形变不同将细胞随机分为4组,分别为0%、8%、12%、16%形变组,每组再按照硝苯地平浓度不同分为4个亚组,分别为0、10、30、50μm亚组。硝苯地平孵育1h后,给予细胞12h静态拉伸,免疫组化染色检测细胞胞浆中MMP-10和MMP-13的表达。结果弹性形变为0%时,各亚组中MMP-10和MMP-13的表达无明显变化,与对照组相比差异无统计学意义(P>0.05)。当硝苯地平浓度为0μm时,8%、12%、16%形变组胞浆内MMP-10和MMP-13均呈高表达,且随形变的增大,表达呈上升趋势(P<0.001);当加入硝苯地平后,随硝苯地平浓度的增加,MMP-10和MMP-13表达均呈下降趋势(P<0.001)。结论硝苯地平对机械力诱导的MMP-10和MMP-13表达有抑制作用,提示钙离子通道参与机械力对HPDLF中MMP-10和MMP-13的诱导表达。

Objective To investigate the effect of Nifedipine on matrix metalloproteinase （MMP）-10 and MMP-13 expression in human periodontal ligament fibroblast（HPDLF） induced by mechanical strain in vitro. Methods HPDLF was divided into four groups at random by mechanical strain of elongation 0%, 8%, 12% and 16%. Each group was divided into four subgroups again by the concentration of Nifedipine 0, 10, 30, 50μm. The cells were imposed to state mechanical strain 12 h after incubated with Nifedipine 1 h. And then immunohistochemical staining was used to investigate the expressions of MMP-10 and MMP-13 in intracytoplasm. Results In 0% group, there was no significant difference of MMP-10 and MMP-13 expression in all subgroups（P〉0.05）. The expressions of MMP-10 and MMP-13 were high in group 8%, 12% and 16% without Nifedipine, and increasing significantly with elongation（P〈0.001）. The expressions of MMP-10 and MMP-13 decreased with dose increasing（P〈0.001） in HPDLF after imposed to Nifidepine. Conclusion The expressions of MMP-10 and MMP-13 induced by mechanical strain were inhibited by Nifedipine, which suggested calcium ions channels participate in expressions of MMP-10 and MMP-13 induced by loading.