豆野螟成虫触角对植物挥发性和性信息素化合物的触角电位反应

Electroantennogram responses of Maruca testulalis (Lepidoptera:Pyralidae) to plant volatiles and sex pheromone

以顺3-己烯乙酸酯为标准化合物,研究了豆野螟雌、雄成虫触角对不同浓度的植物挥发性和性信息素化合物组分的触角电位(EAG)反应.结果表明:豆野螟雌、雄成虫触角对植物挥发性气味具有强烈的EAG反应,但在大部分浓度下,雌、雄虫的反应没有显著差异.在测试的9种植物挥发性化合物中,雌、雄虫对高浓度反-2-己烯醛刺激的相对EAG反应的最大值分别达到250%和260%,且雌、雄虫触角对这种化合物的EAG反应的形状也有很大差异;但对豇豆花气味中的柏木烯和柏木脑没有明显的EAG反应.雌、雄蛾对性信息素都有明显反应,且雌、雄虫的反应存在显著差异,雄虫触角对性信息素化合物主要组分(反10,反12)-十六碳二烯醛的EAG反应的最大值达到250%,远超过雌虫反应的最大值(53%).雌、雄成虫这种神经感觉反应的差异反映了它们在感器结构、功能和行为上的差异.

By using cis-3-hexenyl acetate as standard chemical, the electroantennogram （EAG） responses of Maruca testulalis （Lepidoptera, Pyralididae） to plant volatiles and sex pheromone were investigated. Both the males and the females of M. testulalis showed strong EAG responses to the volatiles, but no significant difference was observed under most test concentrations. Among the test nine plant volatiles, the males and females had greater difference in the shape of EAG response to trans-2-hexenal, and their maximum EAG relative response to high concentration trans-2-hexenal was 250% and 260%, respectively. No obvious EAG responses were observed to the cedrene and cedrol from cowpea Vigna unguiculata floral. Both the male and the female moths showed clear EAG responses to sex pheromone, and significant difference was detected between males and females. The maximum EAG relative response of the males to （E10, E12）-16 CHO, a main component of sex pheromone, was 250%, while that of the females was only 53%. The difference in the EAG responses between the males and females reflected the difference in the structure, function and behavior of the males and females olfactory system.