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水稻叶片淀粉分解同源基因OsSEX1的转录表达

Transcription of Starch Excess(OsSEX1) Homologous in the Rice Leaves
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摘要 通过半定量RT-PCR分析了水稻的SEX1同源基因OsSEX1-1和OsSEX1-2的表达过程,结果表明:2个目的基因表达量均呈现昼夜节律变化。光照对2个基因的表达是必需的,其中以19:00时表达量最高,黑暗条件下表达量较低;叶片淀粉的含量也呈现昼夜节律变化;海藻糖抑制2个基因表达,并导致夜晚叶片淀粉异常积累;在自然光照/黑暗条件下,未观察到不同时段2个基因DNA甲基化差异。 The transcription of two SEX1 homologous（named OsSEX1-1 and OsSEX1-2）in rice leaves were analyzed with semi-quantitative RT-PCR method. Results showed that both genes were of circadian-expression character in rice leaf. Transcripts reached peak at 19：00 hut decreased at night. Light was essential for 2 genes＇ transcription. Starch contents in rice leaf also showed diurnal rhythm. In accordance with the excessive starch accumulation in rice leaf, neither OsSEXI-1 nor OsSEX1-2 expressed when trehalose was used as the only carbon source and no difference of DNA demethylation was detected either in OsSEX1-1 or in OsSEX1-2 at different diurnal stages. The result provided basis for the further study of expression and function of both genes in rice.
作者 金刚 唐向民 汤洪
机构地区 广西大学农学院
出处 《华中农业大学学报》 CAS CSCD 北大核心 2009年第4期381-385,共5页 Journal of Huazhong Agricultural University
基金 国家自然科学基金(30860127) 广西科学基金项目(桂科回0731002) 广西研究生教育创新计划项目(2007105930901M76)资助
关键词 水稻叶片 淀粉 OsSEX1 转录表达 调控 rice leaf starch OsSEX1 transcription regulation
分类号 Q539.1 [生物学—生物化学]
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参考文献13

  • 1YU T S,KOFLER H,HAUSLER R E,et al.The Arabidopsis sex1 mutant is defective in the R1 protein,a general regulator of starch degradation in plants,and not in the chloroplast hexose transporter[J].Plant Cell,2001,13 (8):1907-1918.
  • 2LU Y,GEHAN J P,SHARKEY T D.Daylength and circadian effects on starch degradation and maltose metabolism[J].Plant Physiol,2005,138(4):2280-2291.
  • 3RAMON M,ROLLAND F,THEVELEIN J M,et al.ABI4 mediates the effects of exogenous trehalose on Arabidopsis growth and starch breakdown[J].Plant Mol Biol,2007,63 (2):195-206.
  • 4BAUNSGAARDL,LUTKENH,MIKKELSENR,et al.A novel isoform of glucan,water dikinase phesphorylatea pre-phosphorylated alpha-glucans and is involved in starch degradation in Arabidopsis[J].The Plant Jonrnal,2005,41 (4):595-605.
  • 5YANO R,NAKAMURA M,YONEYAMA T,et al.Starchrelated alpha-glucan/water dikinase is involved in the cold-induced development of freezing tolerance in Arabidopsis[J].Plant Physiol,2005,138(2):837-846.
  • 6RITTE G,LLOYD J R,ECKERMANN N,et al.The starchrelated R1 protein is an alpha-glucan,water dikinase[J].Proc Natl Acad SCI USA,2002,99(10):7166-7171.
  • 7黄越敏,胡红红,武从庆.一个逆境诱导表达的水稻锌指蛋白基因的分离和鉴定[J].华中农业大学学报,2006,25(6):581-585. 被引量：11
  • 8WANG F,SANZ A,BRENNER M L et al.Sucrose synthase,starch accumulation,and tomato fruit sink strength[J].Plant Physiol,1993,101 (1):321-327.
  • 9SINGER S J,LEBON J M,TANGUAY R L,et al.A quantitative Hpa Ⅱ -PCR assay to measure methylation of DNA from a small number of cells[J].Nucleic Acids Rea,1990,18:687.
  • 10PATERSON A H,BOWERS J E,CHAPMAN B A.Ancient polyploidization predating divergence of the cereals,and its consequences for comparative genomics[J].Proc Natl Acad Sci USA,2004,101 (26):9903-9908.

二级参考文献11

  • 1SHINOZAKI K,YAMAGUCHI S K.Molecular responses to dehydration and low temperature:differences and cross-talk between two stress signaling pathways[J].Curr Opin Plant Biol,2000,3(3):217-223.
  • 2LINNEN J M,BAILEY C P,WEEKS D L.Two related localized mRNAs from Xenopus laevis encode ubiquitin-like fusion proteins[J].Gene,1993,128(2):181-188.
  • 3HUANG J,TENG L,LI L,et al.ZNF216 is an A20-1ike and IkappaB kinase gamma-interacting inhibitor of NFkappa B activation[J].J Biol Chem,2004,279 (16):16847-16853.
  • 4YAMAGUCHI S K,SHINOZAKI K.Characterization of the expression of a desiccation-responsive rd29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants[J].Mol Gen Genet,1993,236(2/3):331-340.
  • 5LAITY J H,LEE B M,WRIGHT P E.Zinc finger proteins:new insights into structural and functional diversity[J].Curr Opin Struct Biol,2001,11 (1):39-46.
  • 6TAKATSJI H,MORI M,BENFEY P N,et al.Characterization of a zinc finger DNA-binding protein expressed specifically in Petunia petals and seedlings[J].FMBO J,1992,11(1):241-249.
  • 7LIPPUNER V,CYERT M S,GASSER C S.Two classes of plant cDNA clones differentially complement yeast calcineurin mutants and increase salt tolerance of wild-type yeast[J].J Biol Chem,1996,271(22):12859-12866.
  • 8SUGANO S,KAMINAKA H,RYBKA Z,et al.Stress-responsive zinc finger gene ZPT2-3 plays a role in drought tolerance in petunia[J].Plant J,2003,36(6):830-841.
  • 9MUKHOPADHYAY A,VIJ S,TYAGI A K.Overexpression of a zinc-finger protein gene from rice confers tolerance to cold,dehydration,and salt stress in transgenic tobacco[J].Proc Natl Acad Sci USA,2004,101(16):6309-6314.
  • 10刘强,张贵友,陈受宜.植物转录因子的结构与调控作用[J].科学通报,2000,45(14):1465-1474. 被引量：196

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华中农业大学学报
2009年 第4期

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