荧光定量聚合酶链反应检测c—myc基因在乳腺癌诊断中的应用

Clinical Application of Fluorescent Quantification Polymerase Chain Reaction to Detect c-myc in the Diagnosis of Breast Cancer

目的荧光定量聚合酶链反应（FQ—PCR）技术检测c—myc基因表达，探讨其在乳腺癌诊断中的应用。方法采用FQ—PCR法，并以甘油醛-3-磷酸脱氢酶（GAPDH）为内对照测定15名健康女性体检者、30例良性乳腺疾病患者和80例乳腺癌患者外周血中c—myc的表达量。结果正常对照组和良性乳腺疾病组c—myc和c—myc／GAPDH差异无统计学显著性意义（P〉0．05），乳腺癌组均显著高于前两组，差异有统计学显著性意义（P〈O．05），GAPDH在三组闻差异均无统计学显著性意义（P〉0．05）。若以c—myc表达量和c—myc／GAPDH高于正常对照组为阳性，乳腺癌组的阳性率为41．3％（33／80），良性乳腺疾病组则无1例阳性。27例乳腺癌患者手术前到化疗后第1，28d和56dc—myc和c—myc／GAPDH均呈下降趋势，但第1d和第28d与手术前比较，差异无统计学显著性意义（P〉O．05），而第56d与手术前比较，c—myc和c—myc／GAPDH均显著低于手术前，差异有统计学显著性意义（尸〈O．05）。结论FQ—PCR技术是高灵敏度、高特异度的快速定量检测c—myc方法，可有效监测乳腺癌的诊断、疗效扣转移。

Objective To evaluate the clinical application of fluorescent quantification polymerase chain reaction （FQ-PCR） to detect c-myc in the diagnosis of breast cancer. Methods C-myc in the of clinical samples of 15 health women, 30 patients with benign breast disease and 80 patients with breast cancer were detected by FQ-PCR. GAPDH was used as inter- nal control. Results There were no significant difference in c-myc and c-myc/GAPDH between normal controls and be- nign breast disease group（P〉0. 05）. C-myc and c-myc/GAPDH in breast cancer group were higher than those in the other groups （P〈0. 05）. There were no difference in GAPDH among three groups （P〉0.05）. Using the standard that the ex- pression of c-myc and c-myc/GAPDH was higher than 7~ 2s of normal controls,the positive ratio of breast cancer and be- nign disease were 41.3% and 0 percent respectively. C-myc and c-myc/GAPDH in 27 breast cancer patients were declining after chemotherapy on 1th day,28th day and 56th day. There were no difference among pre-operation and 1th day and 28th day （P〉0. 05）. There was significant difference between pre-operation and 56th day（P（0. 05）. The latter was lower than the former. Conclusion FQ-PCR is a rapid and sensitive and specific method for quantitating c-myc. It also gives objective evidence to the diagnosis,therapy,metastasis of breast cancer.