老年慢性阻塞性肺疾病患者T淋巴细胞免疫状况的研究

Immunological Functions of T-lymphocyte in the Senile Patient with Chronic Obstructive Pulmonary Disease

目的研究老年慢性阻塞性肺疾病（COPD）患者的免疫功能及临床意义。方法采用流式细胞术分别测定57例老年稳定期、63例老年加重期COPD患者和45例正常老年人的T淋巴细胞亚群及各亚群CD25，CD69的水平。结果与正常对照组相比，COPD稳定期患者CD3＋CD4＋／CD3＋CD8＋比值降低（P〈0．05），CD4＋CD25＋，CD8＋CD25＋T淋巴细胞增高（P〈0．05），急性加重期患者CD4＋T淋巴细胞、CD3＋CD4＋／CD3＋CD8＋比值显著降低（P〈0．01），CD3＋CD8＋，CD8＋CD69＋T淋巴细胞增高（P〈0．05），CD4＋CD69叶。T淋巴细胞显著增高（P〈0．01）。急性加重期与稳定期比较，CD4＋T淋巴细胞、CD3＋CD4＋／CD3＋CD8＋比值下降（尸〈0．05）。结论T淋巴细胞亚群和活化参数是观察老年COPD患者T淋巴细胞免疫功能的重要指标，T淋巴细胞可能参与COPD发病及病情发展。

Objective To explore the immunity function and significance of senile patients with chronic obstructive pul- monary disease （COPD）. Methods T lymphocyte subpopulations and CD25,CD69 were assessed by flow cytometry in 57 senile patients with stable COPD, 63 senile patients with COPD during acute exacerbation and 45 health controls. Results Compared with the healthy control ,the ratio of CD3＋CD4＋/CD3＋CD8＋ in stable group was lower （P〈O. 05） ,whereas the percentage of CD4＋CD25＋ and CD8＋CD25＋ T-lymphocyte increased （P〈O. 05）. At the same time ,CD3＋CD4＋ T-lympho- cyte and the ratio of CD3＋CD4＋/CD3＋CD8＋ decreased （_P〈0. 01） in the exacerbated group,CD3＋CD8＋ T-lymphocyte and CD8＋CD69＋ T-lymphocyte increased （P〈O. 05）,CD4＋CD69＋ increased significantly （P〈O. 01）. Compared with the stable group,CD3＋CD4＋ T-lymphocyte and the ratio of CD4＋/CD8＋ in the exacerbated group decreased （P〈O. 05）. Con- clusion T lymphocyte subpopulations and CD25,CD69 are important observational indicators of T-cell immune function in senile patients with COPD. T-lymphocytes may be involved in the onset and development of COPD disease.