血清铜nitro—PAPS终点比色法测定测量点选择的研究

Study on Measuring Point Selection of Serum Copper Determination with Nitro-PAPS Endpoint Methods Influences of Measuring Point Select

目的研究不同测量点设置方式对2-（5-硝基-2-吡啶偶氮）-5-[N-正丙基-N-（3-磺酸丙基）氨基]苯酚二钠（nitro—PAPS）终点比色法测定血清铜结果的影响，以获取准确、可靠的血清铜检验结果。方法分别采用-点终点法、双置二点终点法和单置二点终点法检测样本中铜含量，以质控品、临床标本、精密度和准确度分析结果评价检测效果，应用SPSS13．5统计软件对实验结果进行分析。结果双置二点终点法和单置二点终点法通过质控品评价和临床标本验证且精密度与准确度符合要求，而-点终点法则否。结论nitro—PAPS比色法测量血清铜时，选择双置二点终点法和单置二点终点法测量点设置方式均可获可靠的血清铜检验结果。

Objective To study the influences of different measuring point selection of serum copper determination with 2- （5-nitro-2-pyridylazo） -5-（N-propyl-N-sulfopropylamino） phenol （nitro-PAPS） endpoint methods on serum copper re- suits,aiming at acquiring accurate and reliable laboratory results of serum copper. Methods One-point endpoint method, double-set two-point endpoint method and single-set two-point endpoint method were employed to determine copper con- centration in analytes,analytical results of quality control materials,clinical samples,precisions and accuracieswere applied to assess the test effectiveness,test results were analyzed by utilizing SPSS 13.5 statistical software. Results The analyti- cal results of double-set and single-set two-point endpoint methods passed assessments and their precisions and accuracies were up to the expectations,and one-point endpoint method didn＇ t. Conclusion By selecting double-set and single-set two-point endpoint methods,accurate and reliable results of serum copper determination with nitro-PAPS could be obtained.